目的 探讨香鳞毛蕨有效部位对申克孢子丝菌的体外抑菌作用，进一步明确香鳞毛蕨有效部位的抗菌谱。方法 取4株申克孢子丝菌为受试菌株，培养至酵母相，分别采用琼脂扩散法测定抑菌圈直径和微量稀释法测定最低抑菌浓度(MIC)与最低杀菌浓度(MFC)。结果 香鳞毛蕨有效部位对4株申克孢子菌酵母相的抑菌圈平均直径为25.5 mm，对4株申克孢子丝菌酵母相的MIC几何均数为生药0.18 mg·mL-1，但 MFC>生药20 mg·mL-1。结论 香鳞毛蕨有效部位对申克孢子丝菌酵母相有较强抑菌作用，但无杀菌作用。

Objective To determine antifungal activity of active fraction of Dryopteris fragrans against Sporothrix schenckii Yeast Phase in vitro，and further to clarify it’s antimicrobial spectrum. Methods Four isolates of Sporothrix schenckii were cultured and developed into yeast phase，and then agar diffusion test and microdilution assay were used to obtain the minimum inhibitory concentration(MIC) and minimum fungicide concentration(MFC) value of the four isolates. Results Mean diameter of inhibition zones of 4 isolates of S. schenckii was 25.5 mm，while their geometric mean of MIC was 0.18 mg·mL-1(for the crude herb) and that of MFC was over 20 mg·mL-1(for the crude herb). Conclusion Active fraction of Dryopteris fragrans exerts strong inhibitive effect against S. schenckii，but has no antifungal action in vitro.

R285.5

科技部“十二五”重大新药创制(2011ZX09102-007-03)；广东省科技厅科技计划项目(2010B030700044,2011A030100013)；2010年广东省教育厅高层人才资助项目；广州市科技局科技计划项目(12A062131631)。