目的 采用蛋白质组学方法，在下丘脑中探索琼玉膏对衰老大鼠产生调节作用的相关蛋白质，从而在分子水平上解释抗衰老方剂琼玉膏的作用机理。方法 将2～3月龄42只SD大鼠，随机分为正常对照组、模型组、琼玉膏组，每组14只。给药结束后，抽提下丘脑全蛋白。利用荧光双向差异凝胶电泳、图像分析、胶内酶切、质谱鉴定等蛋白质组学技术，分离、分析、鉴定大鼠下丘脑差异蛋白质；通过数据库检索和生物信息学方法，分析差异蛋白的功能及属性。结果 1. 成功复制亚急性衰老大鼠动物模型；2. 获得了正常对照组、模型组和琼玉膏组下丘脑组织的全蛋白表达荧光双向差异凝胶电泳图谱；3. 运用蛋白质组学技术，比较正常对照组、模型组及琼玉膏组的下丘脑组织，确认了15种表达量发生了变化的蛋白质。结论 鉴定出差异蛋白主要涉及有突触形成相关蛋白、线粒体相关蛋白、能量代谢相关蛋白、抗氧化、细胞骨架蛋白、细胞分化、增殖和凋亡蛋白等，为进一步筛选特异相关蛋白和药物靶位治疗奠定基础。

Objective Proteomics technique was used to investigate the regulatory effect of Qiongyu Extract on the hypothalamic relevant proteins of aging rats，and to expound its anti-aging mechanism on molecular level. Methods Forty-two SD rats aged 2~3 months were equally randomized into 3 groups. Sub-acute aging model was established. After medication for 8 weeks，the whole protein in the rat hypothalamus was extracted for the analysis of differential protein by two-dimensional fluorescence difference gel electrophoresis，proteomics technique，and bioinformatics technique. And then the function and attribute of the differential proteins were analyzed through database search and the method of biological information. Results (1)Sub-acute aging rat model has been established successfully. (2) The two-dimensional fluorescence difference gel electrophoretograms of the hypothalamic whole protein had obtained for the model group，normal control group and Qiongyu Extract group. (3)Fifteen kinds of proteins with differential expression had been identified through the proteomics technique. Conclusion Differential proteins involving synapse formation protein，mitochondrial protein，energy metabolism related protein，antioxidant protein，cytoskeletal protein，and cell differentiation，proliferation and apoptosis protein have been identified，which will supply evidence for the further screening of the disease-specific markers and intervention targets.

R285.5

广东省自然科学基金(10151040701000058)；国家自然科学基金(81173318)。