目的 研究沉香挥发油对H2O₂所致鼠肾上腺嗜铬细胞瘤单克隆细胞系(PC12细胞)氧化损伤的保护作用。方法 体外培养PC12细胞，采用H2O₂制备细胞损伤模型，运用乳酸脱氢酶(LDH)和四唑盐(MTT)法检测细胞活力；应用荧光分光光度法测定细胞膜电位(MMP)和细胞内活性氧含量；应用试剂盒-酶标仪法测定细胞内丙二醛(MDA)含量、超氧化物歧化酶(SOD)和谷胱甘肽还原酶(GSH-Px)的活力。结果 不同浓度H2O₂对PC12细胞生长均有明显的抑制作用，当浓度达到200 μmol·L-1后，对细胞的抑制作用明显减缓；与正常对照组对比较，模型组的细胞存活率较低，ROS、MDA含量较高(P < 0.01)，但SOD和GSH-Px含量明显降低(P < 0.01)；与模型组比较，沉香挥发油各剂量组的ROS、MDA含量明显降低(P < 0.01)，而细胞存活率、SOD和GSH-Px酶活力则显著增强(P < 0.01)。结论 沉香挥发油在一定剂量范围内，对H2O₂致PC12细胞氧化损伤具有保护作用。

Objective To study the protective effects of Lignum Aquilariae Resinatum essential oil on the H2O₂-induced oxidative damage of pheochromocytoma monocloned cell line(PC12 cells). Methods PC12 cells was cultured in vitro，and oxidative damage of PC12 cells was induced by H2O₂. MTT method was performed for the detection of cells viability，fluorospectrophotometry was used to determinate mitochondrial membrane potential(MMP) and the content of intracellular reactive oxygen species(ROS)，and the kit-microplate reader methods were used to determinate malondialadehyde(MDA) content and the activities of superoxide dismutase(SOD) and glutathione reductase(GSH-Px). Results Each concentration of H2O₂ had significant inhibition on PC12 cells，and 200 μmol·L-1 was the maximum inhibitory concentration. The cell viability of the model group was lower，while the contents of ROS and MDA were higher than those of the blank control group(P < 0.01)，and the activities of SOD and GSH-Px were sharply reduced in the model group (P < 0.01). After being treated with Lignum Aquilariae Resinatum essential oil，ROS and MDA were decreased，and the cell viability and the activities of SOD and GSH-Px were increased(P < 0.01 compared with the model group). Conclusion Lignum Aquilariae Resinatum essential oil has protective effect on oxidative damage of PC12 cells in its effective dose range.

R285.5

国家发改委高新技术产业化项目(国发办2011-51)；惠州市科技计划项目(2010BO₂0010005)。