目的 以银杏叶中萜类内酯化合物的含量为指标，评估其在不同环境下的稳定性。方法 将银杏叶在酸、碱、氧化、加热及光照的加速降解条件下放置后，采用HPLC法测定银杏叶中银杏内酯A、B、C和白果内酯共4种萜类内酯的含量。色谱条件:费罗门C18分析柱(4.6 mm × 150 mm，5 μm)；甲醇-水(30 ∶ 70)为流动相；漂移管温度:40 ℃ ；氮气压强3.4 bar。结果 银杏内酯A、B、C和白果内酯分别在0.995～24.875 μg、0.568～14.200 μg、0.489～12.225 μg、0.495～12.375 μg范围内线性关系良好，在酸碱条件，特别是酸性条件下出现降解现象。结论 银杏叶中的萜类内酯在酸及氧化条件下稳定性稍差，储藏时需注意。

Objective To investigate the stability of terpene lactones from ginkgo leaf. Methods Ginkgo leaf was exposed to acids，alkalis，oxidation，heating and light for accelerating the degradation. And then the contents of terpene lactones of ginkgolide A，ginkgolide B，ginkgolide C and blobalide in the compounds were determined by HPLC. The chromatographic conditions were as follows:Diamonsil C18 column(4.6 mm×150 mm，5μm)，mobile phase consisting of Me0H-H2O(30 ∶ 70)，the temperature of drift tube being 40 ℃ and the nebulizer nitrogen pressure at 3.4 bar. Results The reliable liner range of ginkgolide A，ginkgolide B，ginkgolide C and blobalide was 0.995～24.875 μg，0.568～14.200 μg，0.489～12.225 μg，0.495～12.375 μg，respectively. The degradation of ginkgo leaf was obvious especially under acidic condition. Conclusion Terpene lactones from ginkgo leaf show instability in acidic and oxidation conditions，which should be noted during storage.

R284.1