目的 建立HPLC-ELSD法测定更年调和颗粒中黄芪甲苷的含量。方法 色谱柱为Thermo Syncronis C18柱(250 mm×4.6 mm，5 μm)；流动相为乙腈-水(34 ∶ 66)；流速:1.0 mL·min-1；柱温:30 ℃。蒸发光散射检测器(ELSD)参数:漂移管温度85 ℃，氮气体积流量1.8 mL·min-1。结果 黄芪甲苷在0.812~8.12 μg范围内具有良好的线性关系，加样回收率为98.07 %(RSD为1.21 %，n=6)。结论 本法简单易行，重复性符合要求，测定结果准确，可作为更年调和颗粒中黄芪甲苷的含量测定方法。

Objective To establish a HPLC-ELSD method for the determination of astragaloside IV in Gengnian Tiaohe Granula. Methods The HPLC-ELSD system consisted of Thermo Syncronis C18 column(250 mm × 4.6 mm，5 μm) with the mobile phase of acetonitrile-water(34 ∶ 66). The flow rate was 1.0 mL·min-1，and the column temperature was set at 30 ℃. Evaporative light-scattering detector was adopted. The drift tube temperature of ELSD was set at 85 ℃，nitrogen volume flow rate was 1.8 L·min-1. Result Astragaloside IV showed a good linearity in the range of 0.812~8.12 μg. The recovery was 98.07 %(RSD=1.21 %，n=6). Conclusion The established method is simple and reliable，and is suitable for the determination of astragaloside IV in Gengnian Tiaohe Granula.

R284.1

山东省科技发展计划项目(2008GG30002056)；2010-2011年中医药行业科研专项(20110700705)；山东省科技发展计划项目(2011-170)；国家中医药管理局全国名老中医药专家传承工作室建设项目。