[关键词]
[摘要]
目的 建立续断药材中常春藤皂苷元的含量测定方法,并分析不同产地续断中常春藤皂苷元的含量,为全面评价续断药材质量提供参考。方法 采用高效液相色谱分析,Hypersil C18色谱柱,乙腈-0.05 %磷酸(55 ∶ 45)为流动相,检测波长为210 nm。结果 常春藤皂苷元的保留时间为19.22 min,线性范围为0.0122~1.6330 mg·mL-1(r = 0.9999),加样回收率为100.2 %。所测样品中常春藤皂苷元的含量在5.34~45.26 mg·g-1之间。结论 本法能快捷、准确测定不同产地续断药材中常春藤皂苷元,结果显示不同产地含量存在明显差异,可为评价不同产地续断质量提供依据。
[Key word]
[Abstract]
Objective To establish a HPLC method for the analysis of hederagenin in Radix Dipsaci from different habitats,thus to provide the foundation for the quality evaluation of Radix Dipsaci. Methods HPLC was performed on Hypersil C18 column with acetonitrile-0.05 % phosphoric acid(55 ∶ 45) as the mobile phase and detection wavelength was 210 nm. Results The relative retained time of hederagenin was 19.22 min,having a good recovery of 100.2 % and linear correlation in the range of 0.0122~1.6330 mg·mL-1(r=0.9999). The content of hederagenin in Radix Dipsaci from different habitats ranged from 5.34 to 45.26 mg·g-1. Conclusion The method is simple and accurate for the determination of hederagenin in Radix Dipsaci from different habitats. Hederagenin content varies greatly in Radix Dipsaci from different habitats,and the results will provide the foundation for the quality evaluation of Radix Dipsaci from different habitats.
[中图分类号]
R284.1
[基金项目]
广东省广州市生物产业示范工程发展专项资金投资项目(2012-243)。