目的 观察慢性心理应激对2型糖尿病(T2DM)小鼠糖脂代谢及胰岛素抵抗(IR)的影响及加减逍遥散的作用效果。方法 102只自发性糖尿病(KK)小鼠随机分为5组:正常对照(WC)组，模型(SC)组，加减逍遥散对照(XYS)组，应激+加减逍遥散低剂量(LXYS)组及应激+加减逍遥散高剂量(HXYS)组。KK小鼠用高糖高脂饲料喂养8周，采用电击、夹尾、禁食、禁水、合笼饲养等方法复制慢性心理应激模型。检测各组小鼠空腹血糖(FPG)、血清胰岛素(FINS)以及血脂的胆固醇(CHO)、甘油三酯(TG)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)4项等指标，计算胰岛素敏感指数(ISI)。结果 与WC组比较，SC组的ISI明显降低(P <0.01)，体质量明显下降(P < 0.01)，XYS组的FPG明显降低(P < 0.01)、ISI明显增强(P < 0.05)；与SC组比较，LXYS组及HXYS组的ISI明显增强(P < 0.01)，HXYS组体质量明显升高(P < 0.05)，TG，CHO，LDL-C，明显降低(P < 0.01，P < 0.05)，HDL-C，明显升高(P < 0.05)。结论 慢性心理应激能降低T2DM小鼠的胰岛素敏感性，从而加剧其IR以及糖脂代谢的紊乱；加减逍遥散不仅能提高T2DM小鼠的胰岛素敏感性，减轻IR，同时也能对抗慢性心理应激对T2DM小鼠IR的不良影响。

Objective To observe the influences of chronic psychological stress on glycolipid metabolism disorder and insulin resistant(IR) of type 2 diabetes mellitus(T2DM) mice and the intervention effect of Modified Xiaoyao San(XYS). Methods One hundred and two KK mice were randomly divided into 5 groups，normal control group(WC group)，model group(SC group)，T2DM mice treated with XYS group(XYS group)，stress mice treated with low-dose XYS group(LXYS group) and stress mice treated with high-dose XYS group(HXYS group). The KK mice model of T2DM was induced by feeding high-glucose and high-fat forage for 8 weeks，and the model of chronic-psychological- stress was induced by electric shock，clamping the tail，fasting treatment，and raising the male and female mice in the same cage. The observation indexes included fasting plasma glucose(FPG)，fasting insulin(FINS)，total cholesterol(TC)，triglyceride(TG)，low-density lipoprotein cholesterol(LDL-C)，and high-density lipoprotein cholesterol(HDL-C). At last the insulin sensitivity index(ISI) was calculated. Results

R285.5

广东省中医药强省课题(20121230)；广东省教育厅重点实验室课题(AAF433111A50)；广州中医药大学中医药科研创新基金项目(K0100074)。