目的 运用代谢组学方法研究脾气虚证大鼠尿液的代谢表型变化，筛选出与脾气虚证相关的代谢标志物，并探讨补中益气汤的治疗作用。方法 利用核磁共振氢谱(1H-NMR)技术建立大鼠尿液的代谢指纹谱。应用主成分分析(PCA)和正交偏最小二乘判别分析(OPLS-DA)研究正常对照组与模型组、模型组与补中益气汤组之间的代谢物谱差异，并通过变量重要性投影(VIP)在尿液中选取生物标志物。结果 模型组及补中益气汤组的代谢表型发生了明显的变化，并筛选出与脾气虚证相关的9种代谢产物，补中益气汤对部分生物标志物有逆转作用。结论 脾气虚证大鼠的脂质及氨基酸代谢都出现了紊乱，能量代谢降低；经补中益气汤治疗后大鼠蛋白质代谢明显增强，保证了大鼠机体能量的供给。

Objective To study the urine metabolic phenotypes of spleen-qi deficiency in rats for the identification of the corresponding potential biomarkers，and to investigate the therapeutic effect of Buzhong Yiqi Decoction. Methods Proton nuclear magnetic resonance(1H-NMR) technique was used to analyze the rat metabonome. Principal component analysis(PCA) and orthogonal partial least squares discriminant analysis(OPLS-DA) were carried out to investigate the metabonome difference among normal control group，negative control group and Buzhong Yiqi Decoction group. Potential biomarkers in rat serum and urine were selected via the parameter of variable importance in the projection(VIP). Results The metabolic phenotypes of rats varied greatly in negative control group and Buzhong yiqi Decoction group. Nine metabolites in spleen-qi deficiency rat urine were found and Buzhong Yiqi Decoction had reverse effect on some of these biomarkers. Conclusion The lipid and amino acid metabolism are seriously disturbed and energy metabolism is low in rats with spleen-qi deficiency. Treatment with Buzhong Yiqi Decoction can enhance the amino acid metabolism significantly，which ensures the energy supply in rats.

R285.5

广东省自然科学基金项目(S2011010003926)；广东省科技计划项目(2011B061300096,2012B060300031)。