目的 建立毛冬青药材中毛冬青苷B的HPLC测定方法。方法 采用高效液相色谱法，色谱柱:Kromasil C18柱(4.6 mm×250 mm，5 μm)；流动相:以乙腈-0.1 %磷酸梯度洗脱；检测波长为329 nm；流速为1.0 mL·min-1。结果 毛冬青苷B在0.3672~3.672 μg线性关系良好(r=0.9998)，平均回收率为97.26 %，RSD=1.16 %。结论 该方法准确、简便、重现性好，适用于毛冬青苷B的含量测定，有助于完善毛冬青药材的质量控制。

Objective To establish a high performance liquid chromatography(HPLC) method for the determination of pubescenoside B in Radix Ilicus Pubescentis. Methods Kromasil C18 (4.6 mm×250 mm，5 μm)column was adopted. The mobile phase consisted of acetonitrile(A) and 0.1 % phosphoric acid solution(B) in gradient elution mode. The detection wavelength was set at 329 nm. The flow rate was 1.0 mL·min-1. Results The linear range of pubescenoside B was within 0.3672~3.672 μg(r=0.9998). The average recovery was 97.26 %，and RSD=1.16 %. Conclusion The established method is simple，feasible and reproducible. It can be used for the quality control of Radix Ilicus Pubescentis.

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重大新药创制专项“有效部位组方的现代中药冠心康泰临床前研究”(2009ZX09103-360)；广东省国际合作项目(2011B050300021)。