目的 建立香草固齿酊的指纹图谱。方法 采用HPLC法，色谱柱为PLATISIL ODS柱(250 mm×4.6 mm，5 μm)；流动相为乙腈- 0.1 %磷酸溶液，梯度洗脱；检测波长为250 nm；柱温为30 ℃；流速为 0.8 mL·min-1。结果 指纹图谱中共有16个特征峰，其中香附有10个特征峰，墨旱莲有6个特征峰。结论 建立的指纹图谱适用于香草固齿酊的质量控制。

Objective To establish the HPLC fingerprints of Xiangcao Guchi tincture. Methods HPLC was adopted for the fingerprint analysis，and the chromatographic conditions were as follows:PLATISIL ODS(250 mm×4.6 mm，5 μm) column，mobile phase consisting of acetonitrile-0.1 % phosphorous acid solution(gradient elution)，detection wavelength at 250 nm，column temperature being 30 ℃ and flow rate at 0.8 mL·min-1. Results There were 16 common characteristic peaks shown in the fingerprints. Among these peaks，10 peaks were of Rhizoma Cyperi and 6 peaks were of Herba Ecliptae. Conclusion The established fingerprints can be used to control the quality of Xiangcao Guchi tincture.

R284.1

广东省中医药局科研基金(2010202)。