目的 研究灵芝多糖在体外对人类白血病细胞株NB4细胞的增长抑制及凋亡的影响及抗肿瘤的机制。方法 用MTT比色法检测灵芝多糖对NB4细胞增长的抑制作用，Annexin V-FITC / PI双染色法检测NB4细胞的凋亡情况，荧光定量PCR及蛋白印迹技术检测早期生长反应因子1(Egr-1)基因的表达。结果 灵芝多糖对NB4细胞增殖具有抑制作用并可促其凋亡，最高分别为33.5%及18.77%。这种效应呈时间-剂量依赖性。与对照组比较，差异有统计学意义(P < 0.01)。对Egr-1 mRNA和蛋白表达也具有明显的促进作用，差异有统计学意义(P < 0.01)。结论 灵芝多糖在体外能够明抑制NB4细胞的增长并促其凋亡，其机制可能与上调Egr-1的表达相关。

Objective To investigate the effects of Ganoderma lucidum polysacchrides(GLP) on the proliferation and apoptosis of human acute promyelocytic leukemia NB4 cells in vitro，and to explore the possible anti-tumor mechanism. Methods The inhibitory effects of GLP on the proliferation of NB4 cells were measured by using MTT assay. The labeled Annexin V-FITC/PI and flow cytometry were used to detect the apoptotic cells. The expression levels of Egr-1 mRNA and protein were evaluated by fluorescent quantitation PCR and Western blotting，respectively. Results GLP inhibited the proliferation of NB4 cells and promoted its apoptosis in vitro，and the rate being 33.5 %，18.77 % respectively. The effect was time- and dose-dependent(P < 0.01 compared with the control group). The expression levels of Egr-1 mRNA and protein were aslo significantly up-regulated(P < 0.01). Conclusion GLP can inhibit the proliferation of NB4 cells and promoted its apoptosis in vitro，and the mechanism could be related to up-regulation of Egr-1 expression.

R285.5

甘肃省教育厅基金项目(044B-05)。