目的 探讨加味柴胡疏肝散对腹泻型肠易激综合征模型大鼠结肠黏膜胆囊收缩素(cholecystokinin，CCK)的影响。方法 复制大鼠腹泻型肠易激综合征模型，观察加味柴胡疏肝散对模型大鼠粪便含水量、直肠内玻璃小球排出时间以及结肠黏膜中CCK含量(免疫组化法)的影响。结果 与空白对照组比较，模型组大鼠粪便含水量增加，直肠内玻璃小球排出时间缩短，结肠黏膜中CCK的含量升高，差异均有统计学意义(P < 0.05)；加味柴胡疏肝散、匹维溴胺能减轻以上肠功能紊乱(P < 0.05)，降低结肠黏膜中CCK的含量(P < 0.05)。HE染色观察，与空白对照组比较，模型组结肠黏膜未见病理组织学改变。免疫组化显示，与空白对照组比较，模型组CCK染色阳性颗粒明显增加；与模型组比较，加味柴胡疏肝散组及匹维溴铵组CCK染色阳性颗粒减少。结论 加味柴胡疏肝散能减轻腹泻型肠易激综合征模型大鼠的肠功能紊乱，该作用可能与其对结肠黏膜中CCK的调节有关。

Objective To study the effects of modified Chaihu Shugan Powder(MCSP) on cholecystokinin(CCK) level in colonic mucosa of rats with diarrhea-predominant irritable bowel syndrome(D-IBS). Methods With Pinaverium bromide as the positive drug，the effects of MCSP on the water content in the feces，the discharge time of the glass ball from the rectum，and CCK level in colonic mucosa was investigated in D-IBS rats. Results Compared with the blank control group，water content in the feces was increased，the discharge time of the glass ball from the rectum was shortened，and CCK level in colonic mucosa was enhanced in D-IBS rats(P < 0.05). The intervention with MCSP and Pinaverium bromide relieved the intestinal disorder of D-IBS rats，and decreased CCK level(P < 0.05). HE staining showed no pathohistological changes in D-IBS rats，but immunohistochemical assay showed that the number of CCK-positive particles in the colonic mucosa of model group was increased(compared with the blank control group)，while was decreased in MCSP group and Pinaverium bromide group(compared with the model group). Conclusion MCSP has certain effect on relieving the intestinal disorder of D-IBS rats，and the mechanism is probably related with the regulation of CCK level in the colonic mucosa.

R285.5

国家临床重点专科脾胃病科资助项目(国中医药医政发［2012］2号)。