目的 测定杏香兔耳风总酚酸和对照药绿原酸对金黄色葡萄球菌ATCC26003和大肠埃希菌ATCC44102的防耐药变异浓度(MPC)。方法 采用琼脂平板二倍稀释法，测定杏香兔耳风总酚酸和绿原酸对金黄色葡萄球菌和大肠埃希菌的最小抑菌浓度(MIC)、99 %抑菌浓度(MIC99)、初测MPC(MPCpr)和MPC。结果 杏香兔耳风总酚酸、绿原酸对金黄色葡萄球菌的MIC、MPC和细菌耐药选择指数(MPC/MIC99)分别为0.4，6.0 mg·mL-1；10.8，8.4 mg·mL-1；34.0，1.6。对大肠埃希菌的MIC、MPC和MPC/MIC99分别为12.0，6.0 mg·mL-1；16.8，9.6 mg·mL-1；1.4， 1.7。结论 杏香兔耳风总酚酸限制金黄色葡萄球菌耐药突变菌株作用强于绿原酸，限制大肠埃希菌耐药突变菌株作用弱于绿原酸。

Objective To measure the mutation preventing concentration(MPC) of total phenolic acids from Ainsliaea fragrans Champ. and control drug of chlorogenic acid for Staphylococcus aureus ATCC26003 and Escherichia coli ATCC44102. Methods The minimal inhibitory concentration(MIC)，MIC for 99 % of input cells (MIC99)，provisional MPC(MPCpr) and MPC of total phenolic acids from Ainsliaea fragrans and chlorogenic acid for Staphylococcus aureus and Escherichia coli were determined by agar plate doubling dilution method. Results The MIC，MPC and MPC/MIC99 of total phenolic acids from Ainsliaea fragrans for Staphylococcus aureus strain ATCC26003 were 0.34 mg·mL-1，10.8 mg·mL-1，34.0，and those of chlorogenic acid were 6.0 mg·mL-1， 8.4 mg·mL-1，1.6，respectively. The MIC，MPC and MPC/MIC99 of total phenolic acids for Escherichia coli strain ATCC44102 were 12.0 mg·mL-1，16.8 mg·mL-1，1.43，and those of chlorogenic acid were 6.0 mg·mL-1，9.6 mg·mL-1，1.7，respectively. Conclusion The effect of Ainsliaea fragrans total phenolic acids is stronger for restricting Staphylococcus aureus resistant mutants while is weaker for restricting Escherichia coli resistant mutants than that of chlorogenic acid.

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国家自然青年科学基金项目(81102787)；国家“十一五”科技支撑计划(2006BAI06A01-01)；国家“重大新药创制”科技重大专项(2009ZX09103-352)；江西省卫生厅中医药科研计划(2012A158)；江西省自然科学基金重点项目(20133ACB21005)。