目的 建立常用藏药材松蒂(篦齿虎耳草)中总黄酮及绿原酸、芦丁、槲皮素的含量测定方法。方法 以芦丁为对照品，采用分光光度法于491 nm波长处测定篦齿虎耳草中总黄酮的含量；采用Amethyst-C18-P(4.6 mm×250 mm，5 μm)色谱柱，以甲醇-0.4 %甲酸水溶液为流动相梯度洗脱，流速为1 mL·min-1，柱温30 ℃，在350 nm波长处，运用反相高效液相色谱法测定篦齿虎耳草中绿原酸、芦丁和槲皮素的含量。结果 10批篦齿虎耳草样品中总黄酮的平均含量在7 %以上；绿原酸含量为0.2182～0.2849 mg·g-1，芦丁含量为0.3914～0.5982 mg·g-1，槲皮素含量为0.3833～0.7028 mg·g-1。结论 以上两种检测方法简捷、准确、重复性好，可用于篦齿虎耳草药材的质量控制。

Objective To establish a method to determine the contents of total flavonoids，chlorogenic acid，rutin and quercetin in Tibetan medicinal herb“Songdi”(Saxifraga umbellulata var. pectinata). Methods Total flavonoids in Saxifraga umbellulata var. pectinata were determined by UV spectrophotometry at the wavelength of 491 nm，with rutin as standard control. HPLC method was adopted to determine the contents of chlorogenic acid，rutin，quercetin on Amethyst-C18-P Column (4.6×250 mm，5 μm). The mobile phase was methanol-0.4 % formic acid with gradient elution，the flow rate was 1 mL/min，the cloumn was maintained at 30 ℃，and the detecting wavelength was set at 350 nm. Results The average content of total flavonoids was over 7.0% in ten batches of “Songdi” samples. Chlorogenic acid was linear in the content range of 0.2182～0.2849 mg·g-1， rutin in the range of 0.3914～0.5982 mg·g-1，and quercetin in the range of 0.3833～0.7028 mg·g-1. Conclusion The established two methods are simple ，rapid and accurate with good reproducibility ，which are suitable for the quality control of Saxifraga umbellulata var. pectinata.

R284.1

国家科技支撑计划项目子课题(2006DAI06A11-12)。