目的 建立小儿清炎合剂中荭草苷的含量测定方法。方法 采用高效液相色谱法，Hypersil ODS C18(4.6 mm×250 mm，5 μm)色谱柱，以乙腈-0.1%磷酸水溶液(11 ∶ 89)为流动相，流速1.0 mL·min-1，检测波长349 nm，柱温25 ℃。结果 荭草苷线性范围0.1025~1.025 μg，r=0.9999；平均加样回收率为98.12 %，RSD为1.96 %。结论 该方法准确、稳定，重现性好，可用于小儿清炎合剂的质量控制。

Objective To establish a method for the determination of orientin in Xiaoer Qingyan mixture. Methods The determination was carried out by using HPLC method on Hypersil ODS C18(4.6 mm×250 mm，5 μm)column. The mobile phase was a mixture of acetonitrile -0.1 % phosphoric acid(11∶89)，flow rate was 1.0 mL·min-1，detection wavelength was set at 349 nm and column temperature was 25 ℃. Results The linear range of orientin was 0.1025~1.025 μg(r=0.9999)，and the average recovery was 98.12 %，RSD=1.96 %. Conclusion This method is accurate，stable and with good reproducibility，and can be used for the quality control of the Xiaoer Qingyan mixture.

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十一五重大新药创制专项项目(2010ZX09102-214)；江西省自然科学基金项目(2010GQY0045)。