目的 建立Caco-2细胞葡萄糖吸收模型，为考察药物对葡萄糖吸收的影响提供方法。方法 Caco-2细胞培养7，14，21 d后，分别加入25，50，75，100 mmol·L-1的葡萄糖溶液孵育5，10，15，20 min，采用高效液相色谱-质谱联用技术，以稳定同位素示踪法比较培养时间、葡萄糖浓度以及孵育时间对葡萄糖吸收的影响。结果 Caco-2细胞培养21 d后葡萄糖的吸收优于培养7 d和14 d的吸收，吸收量大，趋势稳定。此时用75 mmol·L-1的葡萄糖溶液孵育细胞10 min，葡萄糖吸收量与100 mmol·L-1比较差异无统计学意义，但高于25，50 mmol·L-1时的葡萄糖吸收量。结论 本实验条件下建立的Caco-2细胞模型可作为研究葡萄糖吸收的模型。

Objective To establish Caco-2 cell model of glucose absorption for studying the effect of drugs on glucose absorption. Methods The Caco-2 cells cultured for 7，14 and 21 days were incubated with glucose solution(at 25，50，75 and 100 mmol·L-1) containing stability isotope for 5，10，15 and 20 minutes，respectively. High performance liquid chromatography tandem mass spectrometry was adopted to investigate the effect of culture time，glucose concentration and incubated time on the glucose absorption. Results The glucose absorption volume in Caco-2 cells cultured for 21 days was higher than that for 7 and 14 days，and was in stable tendency. When Caco-2 cells cultured for 21 days were incubated with glucose solution at 75 mmol·L-1 for 10 minutes，the glucose absorption volume was higher than that at 25 and 50 mmol·L-1，but had no significant difference in comparison with that at 100 mmol·L-1. Conclusion The developed Caco-2 cell model can be used for the research of glucose absorption.

R285.5

国家自然科学基金项目(81072753)。