目的 探讨淫羊藿含药血清在骨质疏松大鼠骨髓间充质干细胞(MSCs)成脂分化过程中对过氧化物酶体增殖物激活受体亚型PPARγ mRNA表达的的影响。方法 分离、培养骨质疏松大鼠MSCs，传至3代。将所获细胞随机分为模型组，成脂诱导组，西药组，成脂诱导西药组，中药组，成脂诱导中药高、中、低剂量组。采用淫羊藿含药血清对MSCs进行干预，并与尼尔雌醇含药血清进行对照，观察各组PPARγ mRNA表达的变化。结果 与模型组比较，西药组、中药组PPARγ mRNA表达明显降低(P ＜ 0.01，P ＜ 0.05)；与成脂诱导组比较，成脂诱导西药组、成脂诱导中药高、中、低剂量组PPARγ mRNA表达明显降低(P ＜ 0.05，P ＜ 0.01)。结论 淫羊藿含药血清可抑制绝经后骨质疏松症大鼠MSCs及其在成脂分化过程中PPARγmRNA的表达，从而抑制MSCs成脂分化，以防治骨质疏松症。

Objective To observe the effect of Herb Epimedii on PPARγ mRNA expression of MSCs during adipogenesis in postmenopausal osteoporosis rats. Methods The third generation of MSCs were divided into 8 groups at random，model group(MG)，adipogenic induction group(AIG)，western medicine group(WMG)，adipogenic western medicine group(AWMG)，herb group(HG)，adipogenic low-dose herb group(ALDHG)，adipogenic medium-dose herb group(AMDHG)，adipogenic high-dose herb group(AHDHG). The PPARγ mRNA expression of each group was observed. Results Compared with the MG，PPARγ mRNA expression of WMG and HG were decreased(P ＜ 0.05，P＜0.01). Compared with the AIG， PPARγ mRNA expression of AWMG，AWMG，ALDHG and AMDHG were decreased(P ＜ 0.05，P ＜ 0.01). Conclusion Herb Epimedii can reduce PPARγ mRNA expression of MSCs in postmenopausal osteoporosis rats，inhibit the adipogenesis of MSCs，which contributes to the mechanism of the role of prevention and treatment of osteoporosis.

R285.5

国家自然科学基金资助项目(30901915)；广东省普通高校“中医女性生殖调节与安全性研究”重点实验室开放基金资助项目(ZA201112)；广东省自然科学基金资助项目(S2011010004277)。