目的 观察三七总皂苷对3T3-L1细胞增殖、分化和分泌Leptin、PAI-1的影响，探讨其调脂作用的可能机制。方法 培养3T3-L1细胞，并用不同浓度的三七总皂苷(5，50，100 μg·mL-1)进行干预，以四甲基偶氮唑盐(MTT)法检测细胞增殖；用油红O染色和染色比色法分析脂肪细胞的分化程度，用逆转录多聚酶联反应(RT-PCR)检测脂肪细胞分化相关基因过氧化物酶增殖物激活受体γ2(PPARγ2)、CCAAT/增强子结合蛋白α(C/EBPα)mRNA的表达；用ELISA法检测细胞培养上清中Leptin、PAI-1的含量。结果 三七总皂苷低、中浓度组对模型细胞增殖无明显影响(P ＞ 0.05)，高浓度组能促进细胞的增殖(P ＜ 0.05)；中、高浓度组明显抑制模型细胞的分化，并能抑制PPARγ2、C/EBPα基因的表达(P ＜ 0.01)；药物对模型细胞分泌Leptin、PAI-1有明显抑制作用(P ＜ 0.01)。结论 三七总皂苷可能通过抑制3T3-L1前脂肪细胞的分化和抑制其分泌因子而达到调脂作用，以中剂量作用效果最好。

Objective To investigate the effect of Panax Notoginsenosides(PNS) on the proliferation and differentiation of 3T3-L1 preadipocytes and their secretion of Leptin and PAI-1，and to explore the possible lipid-regulating mechanism. Methods Cultured 3T3-L1 cells were incubated with PNS at the concentrations of 5，50，100 μg·mL-1，and then MTT method was used to detect the cell proliferation，oil red O staining method and spectrophotography were used to analyze the degree of cell differentiation. The expression of adipocytes differentiation-associated genes of peroxisome proliferator-activated receptor γ2(PPARγ2) and CCAAT/enhancer binding protein α(C/EBPα) mRNA was detected by reverse transcription-polymerase chain reaction(RT-PCR). ELISA technique was used to detect Leptin and PAI-1 contents in the culture supernatant of mature fat cells. Results Low- and middle-dose PNS had no effect on the proliferation of 3T3-L1，but high-dose PNS promoted the proliferation of 3T3-L1. Middle- and high-dose PNS inhibited the differentiation of 3T3-L1 and down-regulated the expression of PPARγ2 and C/EBPα mRNA. PNS obviously inhibited the secretion of Leptin and PAI-1 from the model cells. Conclusion PNS can regulate the lipid metabolism through inhibiting the differentiation of preadipocytes and inhibiting the secretion function of the cells，and middle dose has the best action.

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