目的 探讨补骨脂4种组分补骨脂素、corylifol A、新补骨脂异黄酮、补骨脂酚对巨噬细胞RAW264.7细胞炎症因子的影响。方法 ELISA法测定补骨脂4种组分对LPS刺激RAW264.7细胞TNF-a，IL-1β，IL-6分泌的影响及雌激素受体抑制剂(ICI 182.170)的拮抗作用。结果 与正常对照组比较，脂多糖(LPS)组可以显著升高RAW264.7细胞表达TNF-α，IL-1β，IL-6(P < 0.05)； corylifol A、补骨脂酚在10 μmol·L-1浓度下能显著降低TNF-α的分泌(P < 0.05)；补骨脂素、corylifol A、新补骨脂异黄酮在10 μmol·L-1浓度下能显著降低IL-1β的分泌(P ＜ 0.05)；corylifol A、新补骨脂异黄酮、补骨脂酚在10 μmol·L-1浓度下显著降低IL-6的分泌。补骨脂4种组分的抗炎作用与补骨脂雌激素样活性无关。结论 补骨脂4种不同组分对LPS刺激引起的炎症因子的释放有抑制作用，其抗炎作用与雌激素受体样活性无关。

Objective To explore the effects of four components of psoralen，corylifol A，neobavaisoflavone，bakuchiol from Fructus Psoraleae on the inflammatory cytokines in LPS-induced RAW264.7 cells. Methods ELISA was adopted to examine the effect of the four components on the secretion of inflammatory cytokines of TNF-α，IL-1β，IL-6，and on counteracting the inhibition of ICI182.170 by estrogen receptor. Results Compared with the normal control group，TNF-α，IL-1β and IL-6 levels in RAW264.7 cells were significantly increased by LPS(P < 0.05)，TNF-α level was significantly decreased by corylifol A，and bakuchiol at 10 μmol·L-1，and IL-1β secretion was significantly decreased by psoralen，corylifol A and neobavaisoflavone(P < 0.05) at 10 μmol·L-1. Simultaneously，IL-6 expression was also decreased by corylifol A neobavaisoflavone and bakuchiol(P < 0.05) at 10 μmol·L-1. The anti-inflammatory action of the four components from Fructus Psoraleae had no correction with estrogen-like activity of Fructus Psoraleae. Conclusion The four components from Fructus Psoraleae have the inhibitory effect on inflammatory cytokines release in the LPS-induced RAW264.7 cells，and the effect has no correlation with the estrogen receptor.

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科技部国际合作项目(2008DFB30070)；国家自然基金项目(81102860)；天津市卫生局科技基金项目(2010KZ118)。