目的 观察何首乌主要成分二苯乙烯苷(THSG)对去卵巢肾虚大鼠骨髓间充质干细胞(MSCs)增殖及旁分泌细胞因子mRNA的影响。方法 切除SD雌性大鼠双侧卵巢复制肾虚大鼠模型，密度梯度离心法收集各组大鼠MSCs进行体外培养，观察THSG对大鼠第3代MSCs增殖及旁分泌细胞因子mRNA的影响。结果 模型组大鼠第3代MSCs增殖的OD值明显低于正常组(P ＜ 0.05，P ＜ 0.01)，THSG组明显高于模型组(P ＜ 0.05)。模型组MSCs表达粒-巨噬细胞集落刺激因子(GM-CSF)、白血病抑制因子(LIF)、粒细胞集落刺激因子(G-CSF)、骨形成蛋白-2(BMP-2)mRNA明显低于正常组(P ＜ 0.05)；THSG组MSCs表达G-CSF mRNA明显高于模型组(P ＜ 0.05)。结论 与正常大鼠比较，去卵巢大鼠MSCs增殖能力减弱，可能与其MSCs旁分泌GM-CSF、LIF、G-CSF、BMP-2 mRNA减少有关。THSG改善去卵巢肾虚大鼠MSCs增殖能力，可能与其促进MSCs表达G-CSF mRNA有关。

Objective To investigate the effect of 2，3，5，4'-tetrahydroxystilbene-2-O-β-D-glycoside(THSG) on bone marrow mesenchymal stem cells(MSCs) proliferation and paracrine in vitro. Methods The kidney-deficiency animal model was established by ovariectomy on female Sprague-Dawley(SD) rats. After fed with THSG for 3 months，the rats were executed，and MSCs were isolated by density gradient method and purified by whole bone marrow adherent method. The effect of THSG on the proliferation capacity of the third generation of MSCs as well as cytokine mRNA expression was determined. Results The growth curve of the third generation of MSCs showed that MSCs proliferation capacity in the model group was significantly lower than that of normal group and THSG group(P＜0.05 or P＜0.01). At the proliferation phase of MSCs，granulocyte-macrophage colony-stimulating factor(GM-CSF)，leukaemia inhibitory factor(LIF)，granulocyte colony-stimulating factor(G-CSF)，bone morphogenetic protein(BMP-2) mRNA expression level of model group were significantly lower than normal group(P＜0.05)，and G-CSF mRNA expression of model group was significantly lower than THSG group(P＜0.05). Conclusion To some extent，the decreased proliferation capacity of MSCs from ovariectomized rats can be improved by THSG，and the mechanism may be related to the promotion of G-CSF mRNA expression.

R285.5

国家自然科学基金(81202732)；广州中医药大学科研创新基金(11cx058)。