目的 探讨吉祥草及其果实的乙醇提取物及其不同溶剂萃取部位对肿瘤细胞的体外抑制作用，筛选抗肿瘤活性部位。方法 以人肾透明细胞腺癌786-O细胞、人结肠癌HT-29细胞及人肺腺癌A549细胞为供试细胞，采用四甲基偶氮唑盐(MTT)比色法对吉祥草及其果实的不同提取部位进行体外抗肿瘤活性筛选。结果 吉祥草乙醇提取部位、正丁醇分离部位及其果实乙酸乙酯分离部位、正丁醇分离部位对肿瘤细胞均有不同程度的抑制作用，其中吉祥草正丁醇分离部位对786-O、HT-29、A549的细胞毒性较强，其半数抑制浓度(IC50)分别为:92.78，96.04，63.24 mg·L-1，果实正丁醇分离部位对786-O、HT-29、A549细胞有一定的增殖抑制作用，其IC50分别为:179.03，189.67，329.95 mg·L-1。结论 吉祥草正丁醇分离部位是吉祥草主要的抗肿瘤活性部位。

Objective To screen the anti-tumor activity of the extracts from the herb of Reineckia carnea and its fruits. Methods With human kidney clear cell adenocarcinoma 786-O cells，human colon carcinoma HT-29 cells and human pulmonary adenocarcinoma A549 cells as the tested cells，the in-vitro anti-tumor activity of ethanol extract，n-butanol extract and acetoacetate extract isolated from the herb of Reineckia carnea and its fruits was measured by methyl thiazolyl tetrazolium(MTT) colorimetry. Results Ethanol extract and n-butanol extract from the herb of Reineckia carnea，and acetoacetate extract and n-butanol extract from its fruits showed various anti-tumor activity on the tumor cells. The 50 % inhibiting concentration(IC50) of n-butanol extract from the herb of Reineckia carnea against 786-O，HT-29 and A549 cell lines was 92.78，96.04 and 63.24 mg·L-1，respectively；n-butanol extract from the fruits of Reineckia carnea had certain proliferation-inhibitory effect on 786-O，HT-29 and A549 cell lines，the IC50 being 179.03，189.67 and 329.95 mg·L-1，respectively. Conclusion n-butanol extract from the herb of Reineckia carnea is the main anti-tumor active part.

R285.5

江西省科技厅青年科学基金资助项目(20132BAB215033)；江西省卫生厅中医药科研基金项目(2012A132)。