目的 筛选枇杷叶紫珠苯乙醇苷类成分的最佳提取工艺条件。方法 采用反相高效液相色谱法测定枇杷叶紫珠提取液中毛蕊花糖苷及连翘酯苷的含量，以浸膏得率和毛蕊花糖苷及连翘酯苷得率为评价指标，通过正交设计法优选最佳提取工艺。结果 最佳提取工艺为70 %乙醇，料液比为1 ∶ 15，回流提取3次，每次1.5 h。结论 优选出的提取工艺简单可行，结果稳定可靠，可用于提取枇杷叶紫珠苯乙醇苷类成分。

Objective To optimize the extraction technology for phenylethanoid glycosides in Callicarpa kochiana Makino. Methods The content of verbascoside and forsythiaside was determined by high performance liquid chromatography(HPLC). Orthogonal design was used to optimize the extraction technology，and yield rates of total phenylethanoid glycolsides，verbascoside and forsythiaside were adopted as evaluation indexes. Results The optimum technology was circumfluence extracting for three times by 70 % alcohol with solid-liquid ratio at 1∶15，1.5 hours every time. Conclusion The optimum extraction technology is simple and feasible，the results are stable and reliable，and the technology can be used for the extraction of phenylethanoid glycosides in Callicarpa kochiana Makino.

R284.1

国家自然科学基金项目资助课题(81001613)；广东省中医药管理局资助项目(2010396)；广州市珠江科技新星专项(2012J2200038)；广东省大学生创新实验项目(1057210010)。