目的 建立运用HPLC法同时测定肿节风提取物中绿原酸、异嗪皮啶和迷迭香酸含量的方法。方法 采用Agilent ZORBAX Eclipse XDB-C18(4.6 mm × 250 mm，5 μm)色谱柱；流动相:乙腈(A)-0.1 %甲酸(B)，梯度洗脱0～30 min，5 %→20 %A；30～40 min，20 %→25 %A；40～60 min，25 %→29 %A；60～65 min，29 %→32 %A；流速:1 mL·min-1；检测波长:330 nm；柱温:30 ℃；进样量:20 μL。结果 绿原酸、异嗪皮啶、迷迭香酸分别在0.0280~0.448 μg(r = 0.9998)、0.0164~0.262 μg(r = 0.9999)、0.113~1.81 μg(r = 0.9999)范围内呈良好的线性关系；平均回收率(n = 6)分别为103.4 %(RSD = 2.77 %)、98.3 %(RSD = 2.58 %)、95.2 %(RSD = 2.49 %)。结论 该法简便快速，重复性好，准确可靠，可用于同时测定肿节风提取物中绿原酸、异嗪皮啶、迷迭香酸的含量。

Objective To establish a method for simultaneous determination of chlorogenic acid，isofraxidin and rosmarinic acid in the extract of Sarcandra glabra(Thunb.) Nakai. Methods The three components were separated on a Agilent ZORBAX Eclipse XDB-C18 column(4.6 mm×250 mm，5 μm) with a flow-rate of 1 mL·min-1 at 30 ℃. The mobile phase consisted of acetonitrile and 0.1 % formic acid. The gradient condition was A:5 %→20 % from 0 to 30 min，A:20 %→25 % from 30 to 40min，A:25 %→29 % from 40 to 60 min，A:29 %→32 % from 60～65min. The detection wavelength was set at 330 nm and the injection volume was 20 μL. Results The linear ranges of chlorogenic acid，isofraxidin and rosmarinic acid were 0.0280~0.448 μg(r=0.9998)，0.0164~0.262 μg (r=0.9999)，0.113~1.81 μg(r=0.9999)，respectively. The mean recoveries (n=6) of chlorogenic acid，isofraxidin and rosmarinic acid were 103.4 %(RSD=2.77 %)，98.3 %(RSD=2.58 %)，95.2 %(RSD=2.49 %)，respectively. Conclusion The established method is simple，reproducible，and accurate，and can be used for the simultaneous determination of chlorogenic acid，isofraxidin and rosmarinic acid in the extract of Sarcandrae glabra.

R284.1

国家自然科学资金项目(81001662)；国家重点基础研究发展计划(973计划)基金资助项目(2010CB530603)；江西省自然科学基金项目(2009GQY0109)；江西省卫生厅基金项目(2010A099)；江西省教育厅基金项目(GJJ11187)。