目的 建立雷公藤的高效液相指纹图谱，考察雷公藤药材及商品饮片的质量。方法 高效液相色谱条件:色谱柱:Symmetry C18柱(250 mm × 4.6 mm，5 μm)；流动相:乙腈-0.1 %磷酸溶液梯度洗脱；柱温:20 ℃；流速:1.0 mL·min-1；波长:210 nm。对雷公藤种植药材、商品饮片的图谱与HPLC指纹图谱共有模式比较，并进行相似度计算和主成分分析。结果 种植药材的成分分布较为稳定，福建GAP基地药材质量相似度高，商品饮片所含成分与共有模式相比有较大差异，且不同来源饮片之间相互差别明显。结论 根据雷公藤的HPLC指纹图谱可有效地分析和评估药材之间的质量情况，商品饮片质量亟待提高。

Objective To evaluate the quality of commercial and cultivated samples of the root of Tripterygium wilfordii(Leigongteng，LGT) by chromatographic fingerprinting. Methods HPLC conditions were as follow:Agilent 1200 high performance liquid chromatography，Symmetry C18(250 mm× 4.6 mm，5 μm)column mobile phase consisting of acetonitrile-0.1 % phosphoric acid by gradient elution，column temperature at 20 ℃，flow rate being 1.0 mL / min，wavelength at 210 nm. The similarity and PCA analysis were carried out based on the HPLC fingerprint common pattern of LGT. Results The raw material of LGT cultivated in GAP base had stable components distribution，but the ingredients of slices for the decoction varied greatly as compared with the common pattern，some of the slices were inferior. Conclusion The HPLC fingerprint of LGT be used to effectively assess the quality of commercial raw materials，and the fingerprint showed that the quality of slices for decoction needs to be improved.

R284.1

澳门科技基金项目(040/2009/A2)。