目的 建立苦参药材中黄酮类成分的HPLC指纹图谱方法，为科学全面评价该药材质量提供依据。方法 采用HPLC法对不同产地的苦参药材进行分析。色谱条件为Alltech Apollo C18色谱柱(4.6 mm × 250 mm，5 μm)，流动相为甲醇-0.2 %甲酸水溶液系统，梯度洗脱，流速1.0 mL/min，检测波长310 nm，柱温25 ℃。结果 确定了13个色谱峰(或峰组)为各产地药材特征共有峰，并利用对照品对照和LC-MS法对其中的6个共有峰进行了成分指认。结论 该方法简便可靠，重复性好，能够表征苦参药材的内在信息，可用于苦参药材中黄酮类成分的质量控制。

Objective To establish a fingerprint analysis method of flavonoids in herbal medicine of Radix Sophorae by HPLC. Methods The analysis was performed on Alltech Apollo C18 column(4.6 mm×250 mm，5 μm) with a gradient mobile phase of methanol-0.2 % formic acid solution system at flow rate of 1.0mL/min. The detection wavelength was 310 nm. The column temperature was set at 25 ℃. Results The fingerprint of flavonoids in Radix Sophorae was established，and 13 common peaks were obtained. Among them，6 peaks were identified by comparison with the references and by LC-MS. Conclusion The method is quick，simple and reproducible，and can be used as an effective means of quality evaluation of flavonoids in herbal medicine of Radix Sophorae.

R284.1

广东省自然科学基金项目(S2012040006855)；广东高校优秀青年创新人才培养计划项目(LYM10095,2012LYM_0038)；广东省中医药管理局科研项目(20122091)。