目的 建立兔血浆中吴茱萸碱和吴茱萸次碱浓度的测定方法，测定吴茱萸碱和吴茱萸次碱的血浆蛋白结合率。方法 采用平衡透析法测定吴茱萸碱和吴茱萸次碱血浆蛋白结合率，利用HPLC测定血浆中吴茱萸碱和吴茱萸次碱浓度。结果 吴茱萸碱在0.208，1.04，2.08 μg·mL-1浓度下的血浆蛋白结合率分别为(38.5±1.7) %、(36.4±1.0) %、(39.0±1.0) %，吴茱萸次碱在0.235，1.18，2.345 μg·mL-1浓度下的血浆蛋白结合率分别为(40.6±3.2) %、(39.3±4.5) %、(41.5±1.2) %，吴茱萸碱和吴茱萸次碱的浓度对血浆蛋白结合率无显著影响。结论 吴茱萸碱和吴茱萸次碱与兔血浆蛋白具有较低等强度的结合。

Objective To establish an analytical method for evodiamine and rutaecarpine concentration in rabbit plasma，and to determine the plasma protein binding rate of evodiamine and rutaecarpine. Methods The equilibrium dialysis method was adopted to determine evodiamine and rutaecarpine concentration in plasma，and HPLC was used to calculate rabbit plasma protein binding rate of evodiamine and rutaecarpine. Results The protein binding rate of evodiamine was(38.5±1.7)%，(36.4±1.0)%，(39.0±1.0)% when the plasma concentration of evodiamine was 0.208， 1.04，2.08 μg·mL-1，and the protein binding rate of rutaecarpine was(40.6±3.2)%，(39.3±4.5)%，(41.5±1.2)% when the plasma rutaecarpine concentration was 0.235，1.18，2.345 μg·mL-1，respectively. The binding rates of evodiamine and rutaecarpine were not correlated with the plasma concentration. Conclusion Evodiamine and rutaecarpine have a lower protein binding rates with rabbits plasma.

R285.5

江西省科技支撑计划项目(2010JX00095)；南昌市科技支撑计划项目(2010-KJZCJH-YYYYLQX-108)；江西省研究生创新专项资金项目(YC2011-S111)。