目的 建立复方姜黄素脂质立方液晶纳米粒姜黄素和胡椒碱包封率的测定方法。方法 采用HPLC法测定，色谱柱为Kromasil C18柱(4.6 mm × 250 mm，4.6 μm)，流动相为乙腈-0.4 %磷酸(48 ∶ 52)，流速为1 mL·min-1，检测波长为343 nm，430 nm(波长转换时间:16.5 min)；采用微柱离心法和透析法测定包封率。结果 姜黄素在1.8081~18.081 ng范围内线性良好(r=0.9998)；胡椒碱在3.3331～33.331 ng范围内线性良好(r=0.9999)；微柱离心法和透析法测定姜黄素、胡椒碱的包封率分别为59.52 %、56.02 %和60.13 %、55.86 %。结论 采用微柱离心法和HPLC法可以快速、准确地测定复方姜黄素脂质立方液晶纳米粒姜黄素和胡椒碱的包封率。

Objective To establish a RP-HPLC method for the determination of the content and entrapment efficiency of curcumin and piperin in compound curcumin lipid-based liquid crystalline nanoparticles(LCNP). Methods The contents of curcumin and piperin in compound curcumin lipid-based LCNP were determined by HPLC. The column was Kromasil C18(4.6 mm×250 mm，4.6 μm). The mobile phase was acetonitrile-0.4 % phosphoric acid(48∶52) and the flow rate was 1 mL·min-1. The detection wavelength was 343 nm for curcumin and 430 nm for piperin(the converted time set at 16.5 min). The minicolumn centrifugation and dialysis methods were employed to isolate free curcumin and piperine for the entrapment efficiency. Results The curcumin was linear in the range of 1.8081~18.081 ng(r = 0.9998) and the piperine in the range of 3.3331～33.331 ng(r = 0.9999). The entrapment efficiency of curcumin and piperine determined by minicolumn centrifugation was 59.52 % and 56.02 %，and was 60.13 % and 55.86 % by dialysis method，respectively. Conclusion Minicolumn centrifugation combined with HPLC is able to determine the entrapment efficiency of curcumin and piperin in compound curcumin lipid-based LCNP quickly，accurately and conveniently.

R284.1

广东省自然科学基金项目(S2011010005927)；广州市科技计划项目(11C36080689)。