目的 建立地榆药材总皂苷及地榆皂苷-I含量的测定方法，并对不同批次地榆药材中总皂苷和地榆皂苷-I的含量进行测定。方法 采用优化条件的紫外-可见分光光度法建立了地榆药材中总皂苷的含量测定方法，采用HPLC-ELSD法测定地榆药材中地榆皂苷-I的含量。结果 优选出总皂苷含量测定的显色体系为香草醛-冰醋酸-高氯酸，反应条件为15 %香草醛，1.0 mL高氯酸，80 ℃反应15 min，经测定，地榆药材中总皂苷含量为7.25 %~8.41 %；HPLC-ELSD法测得地榆药材中地榆皂苷-I的含量为5.21 %~6.18 %。结论 香草醛-冰醋酸-高氯酸法以及HPLC-ELSD法快速、简便、准确、重现性良好，分别适用于测定地榆药材中总皂苷和地榆皂苷-I的含量。

Objective To establish the quantification method for total saponins and ziyuglycoside I，and to determine their contents in different batches of Sanguisorba officinalis L. Methods The ultraviolet-visible spectrophotometer was optimized for the determination of total saponins，and HPLC-ELSD was employed for the determination of ziyuglycoside I in S. officinalis，respectively. Results The coloration system for the determination of total saponins was vanillin- acetic acid-perchloric acid. The condition of coloration was 15 % vanillin，1 mL perchloric acid，80 ℃ water bathed for 15 min，and the detection wavelength was 549 nm. In seven batches of commercial crude drugs of S. officinalis，the contents of total saponins were measured as 7.25%~8.41 %，and the contents of Ziyu-glycoside I were 5.21 %~ 6.18 %. Conclusion The developed methods are simple，rapid，accurate，stable，and reproducible with high selectivity，and are suitable for the determination of total saponins and ziyuglycoside I in the quality control of S. officinalis，respectively.

R284.1

中山大学医科学生业余科研基金项目([2010]28号)。