目的 建立同时测定三黄颗粒中黄芩苷、小檗碱含量的HPLC法。方法 采用Kromasil C18(4.6 mm × 250 mm，5 μm)色谱柱为固定相，以甲醇-0.1 %磷酸水溶液为流动相，梯度洗脱，流速:1.0 mL·min-1，柱温:25 ℃，进样量:10 μL，检测波长:黄芩苷为280 nm，盐酸小檗碱为345 nm。结果 黄芩苷、小檗碱的线性范围分别为0.2048~2.0480 μg(r = 0.9999)，0.2160～2.160 μg(r = 0.9999)；平均加样回收率分别为99.89 %、99.98 %，RSD分别为1.45 %、1.76 %。结论 本方法操作简便快捷，结果准确可靠，重复性好，可用于三黄颗粒的质量控制。

Objective To establish a HPLC method for the simultaneous determination of baicalein and berberin in Sanhuang Granules. Methods The HPLC analysis was performed on a Kromasil C18 column(250mm×4.60 mm，5 μm)with methanol-water(0.1 % phosphonic acid) as mobile phase in gradient elution. The flow rate was set at 1.0 mL·min-1 and the ultraviolet detector was operated at 280 nm for Baicalein and 345 nm for Berberin，respectively. Results Baicalein and berberin showed good linearity in the range of 0.2048~2.0480 μg(r=0.9999)and 0.2160～2.160 μg(r = 0.9999) respectively. The average recoveries of adding sample were 99.89 % and 99.98 %，respectively and RSD(n = 6) was 1.45 %，and 1.76 %，respectively. Conclusion The established method is simple，accurate and reproducible，which is suitable for the determination of baicalein and berberin in Sanhuang Granules.

R284.1

广东省“211工程”三期重点学科建设项目—药物警戒系统中的中药再评价(粤发改社[2009]972号)。