目的 建立快速高分离度液相色谱(RRLC)同时测定肚痛健胃整肠丸中甘草苷、橙皮苷和甘草酸含量的方法。方法 采用BDS HYPERSIL C18(4.6 mm × 100 mm，2.4 μm)色谱柱；流动相为乙腈-0.2 %磷酸，梯度洗脱；流速为1.0 mL·min-1；检测波长为0～9 min，276 nm；9～14 min，250 nm；柱温为25 ℃；进样体积为4.0 μL，检测器响应时间1 s。结果 样品中甘草苷、橙皮苷和甘草酸在进样量分别为0.0112～0.2800 μg、0.0435～1.0875 μg、0.0630～1.5750 μg范围内有良好的线性关系(r = 0.9999)。平均回收率分别为99.1 %，99.66 %，99.26 %(n=6)；RSD分别为0.79 %，1.61 %，0.99 %。结论 本方法可在较短的时间内快速得到准确的结果，有效控制肚痛健胃整肠丸的产品质量。

Objective To establish a rapid resolution liquid chromatography(RRLC) method for the determination of liquiritin，hesperidin and glycyrrhizic acid in Dutong Jianwei Zhengchang Pills. Methods The separation was performed on BDS HYPERSIL C18(4.6 mm×100 mm，2.4 μm) column. The mobile phase consisted of acetonitrile-0.2 %phosphoric acid with gradient elution at the flow rate of 1.0 mL·min-1. The detection wavelength was 276 nm during 0～9 min and 250 nm during 9～14 min. The column temperature was kept at 25 ℃，injection volume was 4.0 μL，and the response time was 1 s. Results Liquiritin，hesperidin and glycyrrhizic acid were completely separated. The linear ranges of liquiritin，hesperidin and glycyrrhizic acid were 0.0112～0.28μg，0.0435～1.0875 μg and 0.0630～1.575 μg，respectively. The average recoveries(n=6) of liquiritin，hesperidin and glycyrrhizic acid were 99.1 %(RSD=0.79 %) 99.66 %(RSD=1.61 %) and 99.26 %(RSD=0.99 %) respectively. Conclusion The developed RRLC method is simple，rapid，precise and accurate. It can be used for quality control of Dutong Jianwei Zhengchang Pills.

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