目的 建立大鼠血浆中红景天苷及其苷元酪醇的测定方法，并研究其药物动力学。方法 取大鼠10只灌胃给予红景天苷100 mg·kg-1，检测给药前和给药后24 h内红景天苷及酪醇的血浆浓度，并计算其药动学参数。采用液相色谱-串联质谱法，以水杨苷为内标，色谱柱为Alltima C18，流动相:甲醇-水(80 ∶ 20)，等度洗脱，流速为0.3 mL·min-1，柱温为40 ℃，电喷雾负离子源，红景天苷、酪醇和水杨苷的选择检测离子质荷比(m/z)分别为299.2→119.6，137.1→119.0和285.1→122.9。结果 红景天苷和酪醇检测浓度的线性范围分别为50～5000(r=0.9991)、5～500(r=0.9994) ng·mL-1，最低检测限分别为6.25，2.5 ng·mL-1；药动学参数:红景天苷的t1/2β为(5.67±0.84) h，Cmax为(3914.7±915.8)，AUC0→24 h为(8434.2±213.8) ng·h·mL-1；酪醇的t1/2β为(6.24±0.91) h，Cmax为(289.3±44.6) ng·mL-1，AUC0→24 h为(1236.7±73.4) ng·h·mL-1。结论 本方法专属性强、灵敏度高、准确性好，可用于红景天苷和酪醇的血药浓度测定，红景天苷和酪醇在大鼠体内的药动学符合二室模型特征。

Objective To establish a method to determine the plasma concentration of salidroside and its aglycone metabolite tyrosol in rat by using LC-MS/MS，and to calculate the pharmacokinetic parameters of salidroside and its aglycone metabolite tyrosol in rat after oral administration of 100 mg·kg-1 salidroside. Methods Ten rats were given intragastric gavage of 100 mg·kg-1 salidroside，the plasma concentrations of salidroside and tyrosol were detected before administration and within 24 hours after administration，and then the pharmacokinetics parameters were examined. LC-MS/MS was performed on Alltima C18(100×2.0 mm，5 μm)column with salicin as the internal standard. Mobile phase consisted of 80 % methanol-water solution，and flow rate was 0.3 mL·min-1. The temperature of column was 40 ℃. The LC-MS/MS system was operated by using an electrospray ionization probe in the negative ion mode. Scan mode was in multiple reaction ion monitoring(MRM) mode. The ion of monitor was m/z 299.2→119.6 for salidroside，m/z 137.1→119.0 for tyrosol，and m/z 285.1→122.9 for salicin(internal standard)respectively. Results The linear ranges of salidroside and tyrosol were 50 ~ 5000 ng·mL-1(r = 0.9991)and 5~500 ng·mL-1(r = 0.9994) respectively. The lowest limit of quantification(LLOQ) of salidroside and tyrosol were 6.25 ng·mL-1 and 2.5 ng·mL-1. The pharmacokinetic parameters of t1/2β，Cmax，and AUC0→24 h were as follows:(5.67±0.84)h，(3914.7±915.8)ng·mL-1，and (8434.2±213.8)ng·h·mL-1 for salidroside， and(6.24±0.91)h，(289.3±44.6)ng·mL-1，and(1236.7±73.4)ng·h·mL-1 for tyrosol. Conclusion A sensitive，accuracy and suitable LC-MS/MS method for determining salidroside and tyrosol has been developed and successfully applied to the pharmacokinetic study of salidroside after oral administration in rats.

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