目的 观察散瘀镇痛颗粒对寒凝血瘀模型大鼠生殖激素及卵巢血管舒-缩因子的影响，探讨散瘀镇痛颗粒治疗寒凝血瘀型妇科疾病的作用机制。方法 复制大鼠寒凝血瘀模型，散瘀镇痛颗粒混悬液连续灌胃2周，采用放射免疫法测定血清雌二醇(E2)、孕酮(P)、睾酮(T)水平；剖取卵巢，制成匀浆，测定内皮素-1(ET-1)、降钙素基因相关肽(CGRP)活性。结果 与模型组比较，散瘀镇痛颗粒组血清E2、P、T明显升高，卵巢组织匀浆ET-1活性降低，CGRP活性增强，差异有统计学意义(P ＜ 0.05，P ＜ 0.01)。结论 散瘀镇痛颗粒能够调节大鼠卵巢血管舒-缩因子的变化，提升生殖激素水平。

Objective To observe the effect of Sanyu Zhentong Granules on reproductive hormone and ovarian vascular diastolic-systolic factors in rat model of blood stasis due to cold accumulation，and to explore its therapeutic mechanism in treating gynecological diseases. Methods The method of ice water bath was adopted to establish rat model of blood stasis due to cold accumulation. After the suspension of Sanyu Zhentong Granules was given to the rats by intragastrical gavage for 2 weeks in succession，we measured the levels of serum estradiol(E2)，progesterone(P) and testosterone(T) with radioimmunoassay，and detected the activity of endothelin 1(ET-1) and calcitonin gene-related peptide(CGRP) of ovarian homogenate. Results Compared with the model group，the level of serum E2，P and T in Sanyu Zhentong Granules group was obviously increased，while the activity of ET-1 was reduced and CGRP was enhanced in ovarian homogenate. Conclusion Sanyu Zhentong Granules can regulate the rat ovarian vascular diastolic-systolic factors，and increase reproductive hormone levels.

R285.5

江苏省科技支撑计划工业项目(201105007)。