目的 通过研究香附醋制前后对大鼠脊髓c-fos蛋白表达的影响，初步阐明“香附醋制止痛”机理。方法 观察大鼠在疼痛行为学方面的变化；采用免疫组化法检测香附生品与醋制品含药血清对大鼠脊髓c-fos蛋白表达的影响。结果 与模型组比较，香附生品、醋制品均可减少大鼠缩腿、舔爪累计时间，且醋制品作用更强，差异有统计学意义(均P < 0.01)。痛刺激后鞘内注射香附生品及醋制品含药血清均可减少c-fos蛋白表达，与生品比较，醋制品能明显减少c-fos蛋白表达。结论 香附醋制后可通过减少c-fos蛋白表达，阻止痛信号在脊髓内的传导，从而增强镇痛作用。

Objective To investigate the effect of vinegar processed Rhizoma Cyperi on rat spinal c-fos protein expression，for the exploration the theoretical mechanism of vinegar processed Rhizoma Cyperi having analgesic activity in traditional Chinese medicine. Methods The changes of rat pain behavior were observed. The expression of c-fos protein in rat spinal dorsal horn was detected with immunohistochemistry for the evaluation of effects of serum containing crude and vinegar processed Rhizoma Cyperi. Results Serum containing crude Rhizoma Cyperi and serum containing vinegar processing Cyperus could remarkably shorten the time for rats licking paws and lifting the limbs，and serum containing vinegar processing Rhizoma Cyperi had stronger effect(P < 0.01). After injection of serum containing crude Rhizoma Cyperi and serum containing vinegar processing Rhizoma Cyperi into the spinal dorsal horn of rats receiving pain stimulation，spinal c-fos protein expression was obviously decreased，and the serum containing vinegar processing Rhizoma Cyperi had the stronger effect. Conclusion Rhizoma Cyperi after vinegar processing can decrease spinal c-fos protein expression，thus to block the transduction of pain signal in spinal cord，and then enhancing the analgesic effect.

R285.5

江西省自然科学基金(2011GQY0136)。