目的 研究两面针叶不同提取部位的抗炎、镇痛药理活性，为两面针叶的进一步深入研究提供科学依据。方法 采用热板法及扭体法实验考察小鼠镇痛作用，应用小鼠足跖肿胀、耳廓肿胀及棉球肉芽肿胀实验综合评价两面针叶不同提取部位的抗炎活性。结果 与模型组比较，除热板法水部位低剂量的镇痛作用无统计学意义(P < 0.01)外，各剂量的两面针叶粗提取物和不同提取部位均有显著的抗炎和镇痛作用(P < 0.05，P < 0.01)。正丁醇部位在二甲苯所致耳廓肿胀、棉球肉芽肿胀、足跖肿胀等3个抗炎实验和热板法镇痛实验中均显示最强的药理活性，而乙酸乙酯部位在扭体法镇痛试验中活性最强。结论 两面针叶有显著的抗炎、镇痛活性，其中正丁醇和乙酸乙酯提取部位是主要的有效部位。

Objective To study the anti-inflammatory and analgesic activities of extractives with different polarities from the leaves of Zanthoxylum nitidum，and to provide scientific foundation for further study of the leaves. Methods Acetic acid writhing method and hot plate method were used to study the analgesic effect on mice. Auricular swelling induced by dimethylbenzene，cotton-pellet-induced granuloma，and carrageen-induced pedal edema in the mice were observed for the evalutation of anti-inflammatory activites of the extractives. Results Compared with the model control group，water extract of the leaves has been proved to have no significant analgesic activity(P > 0.05)in hot plate experiment，while the crude extract and the other extracting fractions had obvious anti-inflammatory and analgesic activities(P < 0.05，P < 0.01). The ethylacetate extract showed the most potent analgesic activity in acetic acid writhing experiment，and n-butanol extract from the leaves had the most potent effect in all of the anti-inflammatory and analgesic experiments. Conclusion The leaves of Zanthoxylum nitidum show significant anti-inflammatory and analgesic activities，and n-butanol extract and ethylacetate extract have been proved to be the effective fraction.

R285.5

广西自然科学基金项目(2010GXNSFA013187)；广西中医药管理局中医药科技专项课题(GZKZ1141)。