目的 观察怡心健脑颗粒对老年痴呆症(AD)大鼠学习记忆能力的影响，并对其机制进行初步探讨。方法 运用D-半乳糖合β-AP25-35复制大鼠AD模型，水迷宫法观察怡心健脑颗粒对AD大鼠学习记忆能力的影响，并测定大鼠脑组织中丙二醛(MDA)、超氧化物歧化酶(SOD)、乙酰胆碱酯酶(AchE)、过氧化脂质(LPO)的含量；通过制备脑组织切片观察怡心健脑颗粒对学习记忆障碍大鼠海马组织的影响。结果 与正常对照组比较，模型组大鼠的学习记忆能力明显下降，其脑组织中MDA、AchE、LPO含量明显升高，SOD含量明显下降，大脑海马CA3区神经细胞变性坏死；与模型组比较，怡心健脑组大鼠的学习记忆能力明显提高，脑组织中MDA、AchE、LPO含量降低，SOD含量明显升高，CA3区神经细胞病变减轻。结论 怡心健脑颗粒可以提高AD大鼠的学习记忆能力，并能降低脑中MDA、AchE、LPO的含量，减轻AD大鼠脑组织海马神经细胞的病变。

Objective To evaluate the effect of Yixin Jiannao granule on the learning and memory abilities of Alzheimer's Disease(AD) rat model，and to explore its mechanism. Methods D-galactose and β-AP25-35 were injected into rats to establish AD model. Morris water maze test was used to observe the effects of Yixin Jiannao granule on learning and memory abilities of AD rats，and cerebral malondialdehyde(MDA)，superoxide dismutase(SOD)，acetylcholinesterase(AchE) and peroxidative lipid(LPO) contents in the rats were also determined. Slices of brain tissue were prepared for the observation of the histology of AD rat hippocampus. Results Compared with the normal control group，the learning and memory abilities of the model group were decreased significantly，cerebral MDA，AchE，LPO contents significantly were increased，SOD content significantly was declined，and the neurons in hippocampal CA3 showed necrosis and degeneration. Compared with the model group，the learning and memory abilities were improved，MDA，AchE，LPO contents were decreased，SOD content was increased，and the necrosis and degeneration of CA3 neurons had also been relieved in the administration group. Conclusion Yixin Jiannao granule could improve the learning and memory abilities of AD rats，decrease the cerebal content of MDA，AchE and LPO，and relieve the necrosis and degeneration of nerve cells in the hippocampus.

R285.5

浙江省科技厅重点科研社会发展项目(2007C23025)。