目的 观察梓醇对3T3-L1脂肪细胞糖脂代谢及过氧化物酶体增长因子活化受体(PPAR-γ)蛋白表达的影响。方法 采用3T3-L1前脂肪细胞诱导分化为成熟脂肪细胞，以葡萄糖氧化酶法检测细胞的葡萄糖消耗，以油红O染色法检测梓醇、罗格列酮对前脂肪细胞分化的影响，以Western Blot法检测各组PPAR-γ蛋白的表达。结果 与空白对照组比较，梓醇组、罗格列酮组3T3-L1脂肪细胞的葡萄糖消耗量均明显增高(P < 0.01)，梓醇组与罗格列酮组比较差异无统计学意义(P ＞ 0.05)；罗格列酮组OD值及PPAR-γ蛋白的表达较空白对照组明显升高(P < 0.05)；梓醇组OD值及PPAR-γ蛋白的表达较空白对照组明显降低(P < 0.05)。结论 梓醇在抑制PPAR-γ蛋白表达的同时能够促进葡萄糖的消耗，初步表明其具有体外调节脂肪细胞糖脂代谢的作用。

Objective To observe the effect of catalpol on the glucose and lipid metabolism and the protein expression of peroxisome-activated receptor(PPAR-γ) in 3T3-L1 adipocytes. Methods 3T3-L1 preadipocytes were induced to differentiate into mature adipocytes. The effects of catalpol and rosiglitazone on preadipocytes differentiation were evaluated through the examination of glucose consumption by glucose oxidase method，observation of cell differentiation by oil red O staining，and detection of PPAR-γ protein expression by Western Blot analysis. Results Comparing with the blank control group， the glucose consumption in 3T3-L1 adipocytes of catalpol group and rosiglitazone group both significantly increased(P < 0.01)，but the differences between the observation groups were insignificant(P > 0.05). Rosiglitazone group had higher OD value and PPAR-γ protein expression than the blank control group(P < 0.05). On the contrary， OD value and PPAR-γ protein expression of group were significantly decreased(P <0.05). Conclusion Catalpol has inhibitive effect on the protein expression of PPAR-γ and can also promote the glucose consumption of adipocytes，which preliminarily indicates that catalpol would regulate glucose and lipid metabolism in adipocytes cells in vitro.

R285.5

湖北省卫生厅中医药中西结合课题(2006AA301B16)。