目的在欧盟标准EN 13751方法的基础上，建立可区分辐照中药与未辐照中药的光释光法，并对所建方法进行评价。方法将中药样品的测定结果采用欧盟方法与所建方法进行比较，观察系列剂量辐照后的检出情况和掺合稀释后的检出情况，研究光子计数(PSL)值与F值的重复性，研究经辐照处理样品贮藏后的变化情况，研究盲样检出的正确率，以对所建方法进行科学评价。结果样品所含矿物质的种类和数量影响方法的检出灵敏度，敏感度高者低至0.1 kGy的辐照剂量即可以检出，灵敏度低者1 kGy以上的剂量能够检出。在未辐照样品中混入不同比例的经过辐照处理的药粉，掺合比例低至0.1 %的样品也能正确识别。样品10次测定的PSL计数值重复性以及样品3份平行样测得F值的重复性满足要求。盲样测试正确率达100 %。可见，所建方法在灵敏度、准确度、稳定性等各方面均能满足鉴别要求。结论所建光释光法鉴别辐照中药具有正确率高、快速简便的特点，与欧盟方法比较，更适合中药产品，判定规则更加简洁明确，而判定结果的准确度与欧盟方法一致，甚至优于欧盟方法。

Objective To establish a photostimulated luminescence(PSL)method to distinguish irradiated Chinese medicine from the non-irradiated based on European standard of EN 13751，and to evaluate the accuracy of the established method. Methods After irradiation by series of irradiation dose，the samples of Chinese medicine were mixed together with the non-irradiated Chinese medicine，and then were detected by EN 13751 and PSL method respectively. The detection rate of the two methods was compared，and the repeatability between PSL value and F value was studied. The changes of the quality of irradiated Chinese medicine during storage were observed for the study of accurate detection rate for blind samples. Results The detection level of irradiation dose by PSL method was influenced by the sorts of mineral matter and their amount. The detection level of irradiation dose was in the range of 0.1~ 1 kGy. For the mixed Chinese medicine sample，PSL method still worked well even the percentage of non-irradiated Chinese medicine being 0.1%. The reproducibility of results detected by PSL method for 10 times，and F value of PSL method for 3 paralleled samples met the reproducibility requirement. The accuracy for bland sample arrived 100%. The results indicated that the established PSL method met the requirements of irradiated Chinese medicine detection on sensitivity，accuracy，and stability. Conclusion The established PSL method has high accuracy，and is simple and convenient. Compared to EN 13751，the method is suitable for irradiated Chinese medicine for its simpler judgment rule and having the same accuracy of EN 13751.

R284.1

广东省科技计划项目(2010B030700002)。