目的 建立多穗柯提取物中根皮苷、3-羟基根皮苷、槲皮苷、山奈酚-3-O-α-L-鼠李糖苷的含量测定方法。方法采用HPLC法，Dikma PLATISIL TM-C18色谱柱(250 × 4.6 mm，5 μm)；流动相为甲醇(A)-0.2 %磷酸水溶液(B)，梯度洗脱(0 ～ 10 min，35 %A→40 %A；10 ～ 40 min，40 %A→80 %A)，流速:0.8 mL·min-1；柱温:室温25 ℃；检测波长:260 nm。结果根皮苷、3-羟基根皮苷、槲皮苷、山奈酚-3-O-α-L-鼠李糖苷的线性范围分别为0.14～2.91 μg(r=0.9998)，0.82～16.48 μg(r=0.9994)，0.06～1.22 μg(r=0.9999)，0.05～0.98 μg(r =0.9999)；平均回收率分别为100.4 %，99.5 %，98.2 %，97.3 %(n=6)。结论该方法准确、简便，具有良好的重复性和稳定性，适合于多穗柯提取物中4种黄酮苷的含量测定。

Objective To develop a method for simultaneous determination of phloridzin，3-hydroxyl phloridzin，quercetin，kaempferol-3-O-α-L-rhamnoside in the water extract of Folium Lithocarpi Polystachyi. Methods HPLC method was adopted. The analysis was performed on a Dikma PLATISIL TM-C18 column(250 mm×4.6 mm，5 μm)with the mobile phase consisting of methanol(A)-0.2 % phosphate acid(B)by gradient elution(0～10 min，35%A→40%A；10～40 min，40%A→80%A)at a flow rate of 0.8 mL·min-1. The detection wavelength was set at 260 nm and the column temperature was room temperature. Results The linear ranges were 0.14～2.91 μg(r=0.9998)for phloridzin，0.82～16.48 μg(r=0.9994)for 3-hydroxyl phloridzin，0.06～1.22 μg(r=0.9999)for quercetin，and 0.05～0.98 μg(r=0.9999)for kaempferol-3-O-α-L-rhamnoside. The average recoveries(n=6)of four components were 100.4 %，99.5 %，98.2 % and 97.3 %，respectively. Conclusion The developed method is rapid，accurate and with good repeatability. It is suitable for the quantitative analysis of phloridzin，3-hydroxyl phloridzin，quercetin and kaempferol-3-O-α-L-rhamnoside in the water extract of Folium Lithocarpi Polystachyi.

R284.1

广东省科技计划项目(2009A030100014；2011B031700070)。