目的对钩吻根、茎、叶、花、果实生物碱进行薄层色谱研究。方法以钩吻碱甲和钩吻素子为对照，对不同产地钩吻的根、茎、叶、花、果实生物碱进行薄层色谱研究，考察了5种提取方法、4种展开系统、5种薄层板、3种检视方式以及不同温度和湿度对钩吻生物碱薄层色谱的影响。结果以方法5提取，过PXC柱(阳离子交换树脂)，以三氯甲烷-甲醇(9 ∶ 1)为展开系统，稀碘化铋钾试液-10 %三氯化铁乙醇溶液(10 ∶ 1)混合溶液为显色剂，钩吻生物碱薄层色谱信息保留较多，分离较好，薄层效果最佳。结论该方法可作为鉴别钩吻的较专属鉴别方法。

Objective To establish a thin-layer chromatography(TLC)method for the determination of alkaloids in the root，stem，leaf，flower and fruit of Gelsemium elegans Benth. Methods With koumine and gelsemine as the reference substances，5 extraction methods，4 developing systems，5 TLC plates and 3 inspection modes as well as the factors of temperatures and humidity were studied in the assay. Results Method 5 was selected. After purified by PXC column，the samples were developed by the solvent system of CHCl3-MeOH(9 ∶ 1)，with thin potassium heptaiodobismuthate test solution as color-developing agent. The method had much information of alkaloids，and had good resolution and retention. Conclusion The method can be used as a specific TLC identification method for Gelsemium elegans Benth.

R284.1

广州市食品药品监督管理局科技计划项目(2010GZFDA008)。