目的 建立一种准确检测大鼠肝微粒中6-姜酚(6-gingerol)含量的方法。方法以辣椒素为内标物，采用Kcrosmasil-C18柱(4.6 mm × 250 mm，5 μm)色谱柱，以甲醇-水(65 ∶ 35)为流动相，流速1 mL·min-1，检测波长280 nm，进样量20 μL，检测温度为室温。结果6-姜酚浓度在1～100 μg·mL-1浓度范围内与6-姜酚和内标物峰面积之比值有良好的线性关系(r=0.9995)；最低检测限与定量限分别为0.1 μg·mL-1和0.5 μg·mL-1，低、中、高浓度的平均回收率分别为103.5 %，101.1 %，100.6 %，日内RSD分别为3.9 %，6.5 %，1.6 %，日间RSD分别为5.5 %，7.5 %，1.9 %。结论本方法操作简单，快速灵敏，可用于肝微粒中6-姜酚含量的测定。

Objective To develop a HPLC method for the determination of 6-gingerol in rat liver microsomes. Methods Kcrosmasil-C18(4.6 mm×250 mm，5 μm)column was adopted at room temperature. HPLC method was established by using capsaicine as internal standard. The mobile phase consisted of methanol-water(65 ∶ 35) at a flow of 1.0 mL·min-1. The UV detection wave length was set at 280 nm，and the injection volume was 20 μL. Results The liner range of 6-gingerol was 1~100 μg·mL-1(r=0.9995)，the limit of detection was 0.1μg·mL-1 and the limit of quantitation was 0.5 μg·mL-1. The average recovery rates of 6-gingerol at low，medium and high concentrations were 103.5 %，101.1 % and 100.6 %，the intra-day RSD was 3.9 %，6.5 % and 1.6 %，and the inter-day RSD was 5.5 %，7.5 % and 1.9 %，respectively. Conclusion The method is simple，convenient，sensitive for the determination of 6-gingerol in liver microsomes.

R285.5

广东省自然科学基金博士启动项目(10451040701006098)；广州中医药大学创新基金课题(K0090086)。