目的观察制川乌与瓜蒌配伍对慢性心衰大鼠血流动力学的影响，为乌附类中药与瓜蒌配伍应用提供可靠的依据。方法建立大鼠慢性心衰模型，测定川乌相反配伍瓜蒌对心衰大鼠血流动力学指标的影响，并采用链霉素抗生物素蛋白-过氧化酶(S-P)法检测心肌细胞Bcl-2、Bax蛋白表达。结果制川乌与瓜蒌配伍高、中、低剂量组均可改善慢性心衰大鼠血流动力学指标，高剂量组上调Bcl-2抑制凋亡基因的表达，提高Bcl-2/Bax比值。结论适当剂量的制川乌配伍全瓜蒌的共煎液对慢性心力衰竭大鼠心肌具有保护作用。

Objective To study the effect of the combination of antagonism herbs of Radix Aconiti Preparata and Fructus Trichosanthes on the hemodynamics of chronic heart failure rats，thus to provide evidence for its clinical application. Methods We established the rat model of chronic heart failure，observed the changes of hemodynamic parameters，and detected the protein expression of Bcl-2 and Bax in myocardial cells by streptavidin-peroxidase(S-P)immunohistochemical method. Results High-，middle-，and low-dosage combinations of Radix Aconiti Preparata and Fructus Trichosanthes improved the hemodynamic indexes of chroic heart failure in rats. High-dose combination also increased Bcl-2 gene expression，reduced expression of pro-apoptotic gene Bax，and increased Bcl-2/Bax ratio. Conclusion The conbined decoction of antagonism herbs of Radix Aconiti Preparata and Fructus Trichosanthes at appropriate combination proportion has myocardial protective effect in chronic heart failure rats.

R285.5

教育部博士点基金(20102227110002)；吉林省自然科学基金(201015104)。