目的观察瓜子金皂苷己(PGSF)对大鼠原代皮层神经细胞和PC12细胞的保护作用及其机制。方法建立体外氧糖剥夺/复灌(OGD/R)、氧化应激及去血清模型，以MTT法检测细胞存活率，光镜下观察细胞形态，Western blotting法检测蛋白表达。结果PGSF(10，1，0.1μmol·L-1)可明显增加OGD/R、氧化应激和去血清处理细胞的存活率(P < 0.01，P < 0.05)；在OGD/R模型中，PGSF(10、1、0.1μmol·L-1)还可改善神经元形态，减少Caspase-3活性片段的表达(P < 0.01，P < 0.05)，并与MAPK通路抑制剂协同降低神经元死亡率(P < 0.01，P < 0.05)；PGSF(10，1μmol·L-1)可增加神经元Bcl-2/Bax表达比值(P < 0.01，P < 0.05)，并减少p53的表达(P < 0.05)。结论PGSF对OGD/R、氧化应激及去血清导致的神经元或PC12细胞损伤具有保护作用，在OGD/R模型中，该作用机制与其调节凋亡相关蛋白的表达相关，并可能与其抗氧化应激功能有关。

Objective To study the protective effect and related mechanism of polygalasaponin F (PGSF) on primary cultured rat cerebral cortical neurons and PC12 cells. Methods Oxygen-glucose deprivation and reperfusion(OGD/R)，oxidative stress and serum deprivation models in vitro were established respectively. The viability of cells was determined by MTT assay，cell morphology was observed under light microscopy，and the expression of proteins was detected by Western blotting. Results PGSF at the concentration of 10，1 or 0.1 μmol·L-1 could increase the viability of neurons or PC12 cells pretreated by OGD/R，oxidative stress or serum deprivation(P < 0.01 or P < 0.05)，it also improved the morphology of neurons and lowered the expression of cleaved Caspase-3 in OGD/R model (P < 0.01，P < 0.05)，and showed synergistic action on neuron death rate with specific inhibitors of MAPK signal pathway(P < 0.01 or P < 0.05). PGSF at the concentration of 10 or 1 μmol·L-1 could up-regulate the ratio of Bcl-2/Bax proteins(P < 0.01 or P < 0.05) and lower the expression of p53 in neurons injured by OGD/R(both P < 0.05). Conclusion Our studies have revealed the markedly neuroprotective effect of PGSF in OGD/R，oxidative stress or serum deprivation model in vitro. The effect of PGSF is performed partly by regulating the expression of apoptosis-related proteins and is probably associated with antioxidant activity in OGD/R model.

R285.5

国家自然科学基金项目(U0832008,30973887)。