建立高效液相色谱（HPLC）法同时测定黄花蒿水提浸膏中青蒿素、青蒿乙素、猫眼草黄素、猫眼 草酚D 的含量。方法采用Agilent ZORBAX SB-C18（250 mm × 4.6 mm，5 μm）色谱柱；流动相：乙腈（A）- 纯水（B），梯度洗脱；体积流量：0.8 mL·min-1；柱温：30 ℃；进样量：10 μL；检测波长：210 nm。结果青 蒿素、青蒿乙素、猫眼草黄素和猫眼草酚D 进样量分别在1.608 8~16.088 μg（r=0.999 9）、0.014 1~0.141 4 μg （r=1）、0.185 1~1.850 9 μg（r=0.999 9）、0.144 1~1.441 4 μg（r=0.999 9）范围内与峰面积呈良好的线性关系，平均 加样回收率（n=6）分别为102.44%、97.82%、95.07%、95.55%，RSD 分别为1.12%、1.44%、1.29%、1.53%。 结论该方法简便准确、可靠稳定、重复性好，可用于同时测定黄花蒿水提浸膏中青蒿素、青蒿乙素、猫眼草 酚D、猫眼草黄素的含量。

To establish a HPLC method for the simultaneous determination of artemisinin， arteannuin B， chrysosplenetin and chrysosplenol-D in the water extract of Artemisia annua L. Methods The analysis was performed on Agilent ZORBAX SB-C18 （250 mm×4.6 mm， 5 μm） column with a mobile phase of acetonitrile （A） - water （B） and the flow rate of 0.8 mL·min-1 in a gradient elution manner. The column temperature was 30 ℃ . The injection volume was 10 μL， and the detection wavelength was 210 nm. Results Artemisinin， arteannuin B， chrysosplenetin and chrysosplenol-D were correlated well linearly with peak area in their respective ranges 1.608 8-16.088 μg（r=0.999 9），0.014 1-0.141 4 μg（r=1），0.185 1-1.850 9 μg（r=0.999 9），0.144 1- 1.441 4 μg （r=0.999 9），the average recovery rate （n=6）were 102.44%，97.82%，95.07%，95.55%，and the RSD values were 1.12%，1.44%，1.29%，1.53%. Conclusion This method is convenient and accurate. It has good stability and repeatability，and can be used to simultaneously determine the content of artemisinin，arteannuin B， chrysosplenetin and chrysosplenol-D in the water extract of Artemisia annua L.

R284.1

国家自然科学基金项目（82074301）。