目的 探讨玉郎伞水提物对大鼠离体心脏缺血再灌注损伤的作用及其机制。方法 将大鼠随机分为5组，即正常对照组，模型组，玉郎伞水提物低、高剂量组（含生药量3，12 g·L-1），阳性对照药维拉帕米组（含维拉帕米5×10-7 mol·L-1）。离体大鼠心脏采用Langendorff法灌流，停灌30 min再灌30 min造成心肌缺血-再灌注损伤模型。于左心室插入水囊导管，采用MPA心功能分析系统记录心脏血流动力学各项指标，测定冠脉流量（coronary artery flow，CF）和冠脉流出液中肌酸激酶（creatine kinase，CK）、肌酸激酶同工酶（MB isoenzyme of creatine kinase，CK-MB）、乳酸脱氢酶（lactate dehydrogenase，LDH）、乳酸脱氢酶同工酶-1（lactate dehydrogenase isozyme-1，LDH-1）活性以及心肌组织超氧化物歧化酶（superoxide dismutase，SOD）活性、丙二醛（malondialdehyde，MDA）含量。结果 与模型组比较，玉郎伞水提物高剂量组可明显改善缺血-再灌注损伤心功能的各项指标，降低CK、CK-MB、LDH、LDH-1的活性，增加心肌组织SOD的活性，减少MDA的产生。结论 玉郎伞水提物对缺血-再灌注所致的心肌损伤具有保护作用，其作用机制可能与清除氧自由基、抑制脂质过氧化反应有关。

Objective To study the therapeutic effect and mechanism of water extract of Radix Millettia Pulchra（Yulangsan） on myocardial ischemia-reperfusion injury in isolated rat hearts. Methods Rats were randomized into 5 groups，namely normal control group，model group，Verapamil group（5×10-7 mol·L-1），and low- and high-dosage Yulangsan water extract groups（at the concentration of 3，12 g·L-1）. Langendorff-perfusion method was used for the establishment of isolated rat heart model of myocardial ischemia reperfusion injury by global ischemia for 30 minutes and then reperfusion for 30 minutes. The effect of Yulangsan water extract on cardiac hemodynamics was observed by MPA cardiac function system（CFS）. The coronary artery flow（CF） was monitored，and the activities of creatine kinase（CK），MB isoenzyme of creatine kinase（CK-MB），lactate dehydrogenase（LDH），lactate dehydrogenase isozyme-1（LDH-1）in the effluent from coronary artery were measured. Meanwhile，the activities of superoxide dismutase（SOD） and the content of malondialdehyde（MDA） in the myocardial tissues were determined. Results Yulangsan water extract at 12 g·L-1 could recover cardiac function，and reduce the activities of CK，CK-MB，LDH and LDH-1. It could increase the activity of SOD，and decrease the MDA product. Conclusion Yulangsan water extract exerts myocardial protective effects against ischemia reperfusion injury，and its mechanism may be related to the effects on scavenging oxygen free radical and reducing lipid peroxidation.

R285.5

广西科技基础条件平台建设（12-97-20，10-108-19）；广西自然科学基金重点项目（2011GXNSFD018030）；南宁市科技攻关与新产品试制（201102084C）；南宁市青秀区科学研究与技术开发计划（2013S12）；2011年度留学人员科技活动项目择优资助（人发[2001]33号）。