目的 观察王氏连朴饮对脾胃湿热证模型大鼠胃黏膜增殖与凋亡相关P53、Bcl-2和COX-2蛋白表达的影响，探讨该方治疗脾胃湿热证获效的可能作用机制。方法 取SD大鼠50只，随机分为正常对照组，模型组，王氏连朴饮高、中、低剂量组（1.94，0.97，0.48 g·mL-1），共5组，每组10只。除正常组饲以普通饲料外，其余4组均以湿热环境加高脂高糖饮食和白酒综合法复制脾胃湿热证模型，连续灌胃给药7 d，观察该方对模型大鼠胃黏膜P53、Bcl-2及COX-2蛋白表达的影响。结果 本实验P53、Bcl-2、COX-2基因蛋白表达定位于胞质，模型组大鼠表达明显升高，与正常对照组比较，有显著性差异（P＜ 0.01）。王氏连朴饮高、中、低剂量组P53、Bcl-2及COX-2蛋白表达有一定程度下调，与模型组比较，具有显著性差异（P＜ 0.01），尤以中剂量组作用最明显。结论 王氏连朴饮可能通过下调脾胃湿热模型大鼠胃黏膜P53、Bcl-2及COX-2蛋白水平的表达，在一定程度上调节其胃黏膜细胞增殖与凋亡的失衡，进而达到对胃黏膜损伤的修复作用。

Objective To observe the effect of Wang's Lian Pu Decoction（WLPD） on protein expression of proliferation and apoptosis correlated P53，Bcl-2，COX-2 genes in gastric mucosa of rat model wiht splenogastric damp-heat syndrome（SDHS），and to explore its possible therapeutic mechanism for SDHS. Methods Fifty Sprague Dawley rats were evenly divided into normal control group，model group，and high-，middle- and low-dosage WLPD groups（1.94，0.97，0.48 g·mL-1）. SDHS rat model was established by the combined method of damp-heat environment and feeding with high fat and sugar diet and wine. The intervention with gastric gavage of WLPD was given simultaneously together with the modeling. The treatment lasted 7 days，and the effects of WLPD on protein expression levels of P53，Bcl-2 and COX-2 genes were observed. Results The protein expression of p53，Bcl-2 and COX-2 genes was localized in cytoplasm and their expression levels were significantly increased in the model group as compared to normal control group（P＜ 0.01）. High-，middle- and low-dosage WLPD showed an effect on down-regulating the protein expression of P53，Bcl-2 and COX-2 genes to certain extent，and the middle-dose had the strongest effect，the difference being significant compared with the model group（P＜ 0.01）. Conclusion WLPD may down-regulate the protein expression of P53，Bcl-2 and COX-2 genes in gastric mucosa of SDHS rats，correct the imbalance of the proliferation and apoptosis of gastric mucosa cells，thus has protective effect on the gastric mucosa.

R285.5

贵州省科技厅-贵阳中医学院联合基金资助项目（黔科合中药字[2012]LKZ7023）。