目的 建立地埝药材中没食子酸的RP-HPLC测定方法。方法 采用高效液相色谱法，色谱柱：Cosmosil C18（4.6 mm×250 mm，5 μm）；流动相为甲醇-0.5 %磷酸溶液；检测波长为274 nm；流速为1.0 mL·min-1；柱温为25 ℃。结果 没食子酸在0.234～1.794 μg内线性关系良好（r=0.9999），平均回收率为99.33 %，RSD=1.19 %。结论 该方法准确、简便、重现性好，适用于没食子酸的含量测定，有助于完善地埝药材的质量控制方法。

Objective To establish a method for the determination of gallic acid in Melastoma dodecandrum Lour. from different sources by RP-HPLC. Methods HPLC method was validated on Cosmosil C18（4.6 mm×250 mm，5 μm）with a mobile phase of methanol -0.5 % phosphoric acid at a flow rate of 1mL/min and a column temperature of 25 ℃. The detection wavelength was set at 274 nm. Results Gallic acid in the range of 0.234~1.794 μg（r=0.9999） had a good linear relationship，the average recovery was 99.33 %，and RSD=1.19 %. Conclusion The established method is simple，feasible and reproducible，abd it can be used for the quality control of Melastoma dodecandrum Lour.

R284.1

《中华人民共和国药典》2015版一部标准研究项目。