目的 确定柴胡加龙骨牡蛎汤改善围绝经期睡眠的有效部位及最佳给药剂量。方法 以去卵巢合并电刺激小鼠为动物模型，观察柴胡加龙骨牡蛎汤水洗脱部位和不同浓度乙醇洗脱部位对戊巴比妥钠协同睡眠时间，以及对模型小鼠子宫系数和肾上腺系数的影响。结果 与模型组比，柴胡加龙骨牡蛎汤70%醇洗脱组（48 mg·kg-1）可以明显延长模型小鼠的睡眠时间（P＜0.01），增加模型小鼠的子宫系数（P＜0.05）。结论 柴胡加龙骨牡蛎汤改善睡眠作用的有效部位为70 %醇洗脱部位，最佳给药剂量每天为48 mg·kg-1。

Objective To screen the effective parts and optimal dose of Chaihu Jia Longgu Muli Decoction，a Chinese herbal recipe composed of Longgu Muli Decoction with Radix Bupleuri for improving sleep of perimenopausal female mice. Methods Ovariectomy and electrical stimulation were given to the female mice to the model. And then，we observed the effects of water elution part of Chaihu Jia Longgu Muli Decoction and the elution parts obtained by elution with different concentrations of ethanol on pentobarbital-induced sleep time of the model mice，and the effects on uterus coefficient and adrenal gland coefficient of the model mice. Results Compared to the model group，70 % ethanol（48 mg·kg-1） elution of Chaihu Jia Longgu Muli Decoction had significant effect on prolonging the pentobarbital- induced sleep time（P＜ 0.01） and on increasing the uterus coefficient（P＜ 0.05） of the model mice. Conclusion The part of Chaihu Jia Longgu Muli Decoction eluted by 70 % ethanol is the effective part for improving sleep of perimenopausal female mice，and the optimal dose is 48 mg·kg-1 per day.

R285.5

黑龙江省普通高等学校青年学术骨干支持计划（1254G054）；黑龙江中医药大学校科研基金（2012bs01）；黑龙江中医药大学“优秀创新人才支持计划”（2012RCD02）；教育部重点实验室-北药基础与应用研究开放课题基金资助项目。