目的 建立HepG2细胞胰岛素抵抗模型，并用于评价滇越金线兰中2个丁酸衍生物对胰岛素敏感性的改善作用。方法 采用高糖高浓度胰岛素诱导 HepG2细胞建立肝细胞胰岛素抵抗模型，研究滇越金线兰2个丁酸衍生物对胰岛素抵抗模型细胞葡萄糖摄取和细胞活力的影响。结果 胰岛素浓度为10-7 mol·L-1时细胞的葡 萄糖摄取量最小，为合适的模型复制浓度。与模型组比较，2个丁酸衍生物都能显著增加胰岛素抵抗的HepG2细胞对葡萄糖的吸收，葡萄糖摄取量分别增加了76.7 ％和77.8 ％，半数有效浓度（EC50）分别为9.372和10.854 μmol·L-1，对胰岛素抵抗的HepG2细胞的葡萄糖摄取呈现较好的促进作用。结论 2个丁酸衍生物对胰岛素具有增敏活性， 能有效促进胰岛素抵抗细胞对葡萄糖的摄取和利用。

Objective To establish an insulin-resistance model of HepG2 cells and to investigate the effect of two natural butanoic acid derivatives from Anoectochilus chapaensis on insulin resistance. Methods HepG2 cells were cultured in Dulbecco’s modified Eagle’s medium（DMEM ） supplemented with high glucose and high-concentration insulin to optimize the conditions for the establishment of insulin-resistance cell model. Glucose consumption in the cells was determined by glucose oxidase method. The effects of the tested samples at different concentrations on the vitality of the insulin resistant cells were evaluated by methyl thiazolyl tetrazolium（MTT） staining. Results Insulin resistance cell model was induced successfully when the insulin concentration was at 10-7 mol·L-1. Compared with the model group，the glucose uptake was significantly increased by 76.7 ％ and 77.8 ％，and the EC50 was 9.372 and 10.854 μmol·L-1 in groups treated with metformin and the butanoic acid derivatives（P＜ 0.01）， respectively. Conclusion The two natural butanoic acid derivatives from Anoectochilus chapaensis may be good potential candidates focusing on improving the state of insulin resistance，and promoting the intake and utilization of glucose by the insulin-resistance HepG2 cells.

R285.5

广东省医学科研基金项目（B2011150）；国家自然科学基金项目（81001628）；广东省自然科学基金项目（S2013010014771）。