目的 建立一种便捷灵敏的青蒿素含量测定方法，并测定其在不同溶剂中的溶解度及油水分配系数。方法 均匀试验设计优化青蒿素的柱前衍生条件，采用HPLC-ECD法进行测定。结果 优化的青蒿素柱前衍生条件为：1 mL青蒿素无水乙醇溶液与4 mL氢氧化钠0.01 mol·L-1于70 ℃水浴中反应50 min后，冷却至室温，再加入5 mL醋酸0.06 mol·L-1。青蒿素在0.0015~0.7500 μg范围内线性关系良好。结论 建立的柱前衍生HPLC-ECD法专属性强、灵敏度高，适用于青蒿素的测定。

Objective To establish a sensitive assay method for artemisinin，and to detect the solubility as well as its oil-water partition coefficient in different solvents. Methods The pre-column derivatization of artemisinin was optimized by uniform design，and the determination of artemisinin was performed by HPLC-ECD. Results The optimal pre-column derivatization conditions were as follows：one milliliter of artemisinin anhydrous ethanol solution and 4 mL 0.01 mol·L-1 sodium hydroxide were reacted in 70 ℃ water bath for 50 min，and then cooled to room temperature with 5 mL of 0.06 mol·L-1 acetic acid added. The content of artemisinin showed good linearity in the range of 0.0015~0.7500 μg. Conclusion The pre-column derivatization HPLC-ECD method is highly specific and sensitive，and can be used for the assay of artemisinin.

R284.1

中柬第三方检测平台建设项目（2012年广州市白云区国际科技合作计划项目）（2012-KQ-23）。