目的 研究芪龙头痛颗粒(Qilong Toutong granule，QLTT)的镇静作用、活血化瘀功效及其抗偏头痛机制。方法 实验动物随机分为6组:即正常对照组，正天丸组，模型组及QLTT高、中、低剂量组。72只昆明小鼠灌胃给药3 d后，用自主活动实验和协同催眠实验观察QLTT的镇静催眠作用。60只SD大鼠灌胃给药7 d后，皮下注射盐酸肾上腺素注射液2次，期间冰水浸泡5 min复制急性血瘀模型，禁食12 h后取血检测血黏度。60只昆明小鼠经利血平皮下注射9 d复制低5-羟色胺(5-HT)偏头痛模型，从模型复制第3天起，给予相应药物进行灌胃，末次灌胃1 h后，取血与脑组织进行凝血时间和单胺类递质的测定。结果 QLTT高、中、低剂量组与正天丸组自主活动次数减少，提示具有明显的镇静作用(P＜0.05，P＜0.01)，但无催眠作用；可显著改善血瘀模型鼠的血液黏度(P＜0.01)；增加脑组织5-HT、多巴胺(DA)和去甲肾上腺素(NE)的含量(P＜0.05，P＜0.01)；QLTT高、中剂量组和正天丸组能显著延长低5-HT偏头痛模型小鼠的凝血时间(P＜0.01)；QLTT中剂量组和正天丸组可提高血浆5-HT水平(P＜0.05)。结论 QLTT具有明显的镇静作用和活血化瘀功效，可能是通过提高血浆和脑组织的5-HT、NE和DA的含量，抑制三叉颈复合体神经元的活化，从而减轻偏头痛的疼痛程度。

Objective To study the sedative effect and antimigraine mechanism of Qilong Toutong granules(QLTT)，as well as its actions of activating blood and dissolving stasis. Methods Experimental animals were randomly divided into 6 groups，namely blank group，Zhengtian pills(ZTP)，diazepam group(or model group)，and high-，medium- and low-dose QLTT groups. Seventy-two Kunming mice were given adaptive intragastric gavage for 3 days，and then spontaneous locomotor test and sodium pentobarbital-induced hypnosis activity were adopted to estimate the sedative effect. After adaptive gavage for 7 days，60 SD rats were given adrenaline subcutaneously twice，and were kept in ice water for 5 min every time to induce acute blood stasis. After fasting for 12 h，blood samples of SD rats were collected for detecting blood viscosity. In addition，60 Kunming mice were administered reserpine subcutaneously for 9 days to induce 5-hydroxytryptamine(5-HT) type of migraine. From the third modeling day，experimental animals were given the corresponding medicine by intragastric gavage. One hour after the last administration，blood and brain tissue samples were obtained，and then blood clotting time，and the monoamine neurotransmitter levels of brain tissue and plasma were detected. Results The high-，medium- and low-dose of QLTT and ZTP all had remarkable sedative effect(P＜0.05，P＜0.01)，but had no hypnotic effect. And they could obviously reduce blood viscosity of blood stasis model rats(P＜0.01) and increase 5-HT，dopamine(DA) and noradrenalin(NE) contents of brain tissue and plasma(P＜0.05，P＜0.01). The high- and medium-dose of QLTT and ZTP could prolong the blood clotting time of mice with 5-HT type of migraine(P＜0.01)，and medium-dose of QLTT and ZTP could enhance plasma 5-HT levels(P＜0.05). Conclusion QLTT have remarkable sedative effect and potent actions of activating blood and dissolving stasis. The antimigraine mechanism of QLTT is probably related with the increase of cerebral and plasma 5-HT，NE and DA contents，and with the inhibition of activation of trigemino-cervical complex neurons.

国家科技支撑计划项目居家养老健康服务内容建设(2013BAH14F03)。